Table 1 List of the internal reference genes and the amplification specifications in qRT-PCR
No | Gene symbol | Gene name | GenBank accession No. | Primer sequence (5′-3′), Fwd // Rev | Amplicon size (bp) |
|---|---|---|---|---|---|
1 | 18S | 18S ribosomal RNA | AF069218.1 | CTACGTCCCTGCCCTTTGTACA// ACACTTCACCGGACCATTCAA | 213 |
2 | 25S | 25S ribosomal RNA | M11585.1 | AAGGCCGAAGAGGAGAAAGGT// CGTCCCTTAGGATCGGCTTAC | 238 |
3 | ACT | Actin | AB047313.1 | CAGCCACACTGTCCCCATCTA// AGCAAGGTCGAGACGAAGGA | 184 |
4 | β -TUB | β – tubulin | D30716.1 | GCTGACCACACCTAGCTTTGG// AGGGAACCTTAGGCAGCATGT | 224 |
5 | eEF-1a | Eukaryotic elongation factor 1 - alpha | GQ848073.1 | TTCACTCTTGGTGTGAAGCAGAT// CTTCCTTCACGATTTCATCGTAA | 228 |
6 | eIF-4a | Eukaryotic initiation factor 4 - alpha | AB046414.1 | TTGTGCTGGATGAAGCTGATG// GGAAGGAGCTGGAAGATATCATAGA | 236 |
7 | UBC | Ubiquitin-conjugating enzyme E2 | AK059694 | CCGTTTGTAGAGCCATAATTGCA// AGGTTGCCTGAGTCACAGTTAAGTG | 188 |
8 | UBQ-5 | Ubiquitin 5 | AK061988.1 | ACCACTTCGACCGCCACTACT// ACGCCTAAGCCTGCTGGTT | 167 |
9 | UBQ-10 | Ubiquitin 10 | AK101547 | TGGTCAGTAATCAGCCAGTTTGG// GCACCACAAATACTTGACGAACAG | 192 |
10 | GAPDH | Glyceraldehyde – 3 – phosphate dehydrogenase | GQ848049.1 | AAGCCAGCATCCTATGATCAGATT// CGTAACCCAGAATACCCTTGAGTTT | 189 |