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Table 2 Mean Ct values and amplification efficiency of candidate internal reference genes

From: Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

Gene symbol Water Xoo MT-Water MT-Xoo PCR efficiency R2
Mean (Ct) SD(±Ct) Mean (Ct) SD(±Ct) Mean (Ct) SD(±Ct) Mean (Ct) SD(±Ct)
18S 7.509022 0.057528 7.541728 0.151079 7.364891 0.140951 7.251798 0.063727 1.9935 0.99725
25S 9.759871 0.102621 9.831956 0.09594 9.436561 0.210717 9.561671 0.235636 1.91625 0.999
ACT 18.79102 0.221776 19.12131 0.191417 18.35688 0.266497 19.43089 0.171603 1.83575 0.9715
β-TUB 28.43714 0.176962 28.56222 0.206912 28.43466 0.369246 28.94232 0.482744 1.8635 0.99975
eEF1-a 17.3651 0.059846 17.68532 0.065735 17.86457 0.250979 18.01716 0.289779 1.8835 0.99975
eIF-4a 20.72344 0.109694 20.98324 0.058178 21.19292 0.312464 21.439 0.314081 1.87125 0.99975
UBC 21.93981 0.165087 22.1574 0.05665 22.20476 0.223596 22.37866 0.215301 1.87825 0.9995
UBQ5 17.94632 0.099612 18.21766 0.077023 18.40336 0.207519 18.63075 0.212819 1.8825 0.99975
UBQ10 18.2794 0.152598 18.5252 0.088786 18.87545 0.2442 19.2134 0.262454 1.885 0.99975
GADPH 26.33569 0.183057 26.26842 0.216105 26.69255 0.298437 26.88369 0.329891 1.86925 0.99925