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Table 2 Mean Ct values and amplification efficiency of candidate internal reference genes

From: Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

Gene symbol

Water

Xoo

MT-Water

MT-Xoo

PCR efficiency

R2

Mean (Ct)

SD(±Ct)

Mean (Ct)

SD(±Ct)

Mean (Ct)

SD(±Ct)

Mean (Ct)

SD(±Ct)

18S

7.509022

0.057528

7.541728

0.151079

7.364891

0.140951

7.251798

0.063727

1.9935

0.99725

25S

9.759871

0.102621

9.831956

0.09594

9.436561

0.210717

9.561671

0.235636

1.91625

0.999

ACT

18.79102

0.221776

19.12131

0.191417

18.35688

0.266497

19.43089

0.171603

1.83575

0.9715

β-TUB

28.43714

0.176962

28.56222

0.206912

28.43466

0.369246

28.94232

0.482744

1.8635

0.99975

eEF1-a

17.3651

0.059846

17.68532

0.065735

17.86457

0.250979

18.01716

0.289779

1.8835

0.99975

eIF-4a

20.72344

0.109694

20.98324

0.058178

21.19292

0.312464

21.439

0.314081

1.87125

0.99975

UBC

21.93981

0.165087

22.1574

0.05665

22.20476

0.223596

22.37866

0.215301

1.87825

0.9995

UBQ5

17.94632

0.099612

18.21766

0.077023

18.40336

0.207519

18.63075

0.212819

1.8825

0.99975

UBQ10

18.2794

0.152598

18.5252

0.088786

18.87545

0.2442

19.2134

0.262454

1.885

0.99975

GADPH

26.33569

0.183057

26.26842

0.216105

26.69255

0.298437

26.88369

0.329891

1.86925

0.99925