Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

Table 3 Gene expression stability of 10 candidate reference genes calculated by Bestkeeper

Gene symbol	Water			Xoo			MT-Water			MT-Xoo
Gene symbol	n^a	SD (± Ct)	CV [% Ct]^b	n^a	SD (± Ct)	CV [% Ct]^b	n^a	SD (± Ct)	CV [% Ct]^b	n^a	SD (± Ct)	CV [% Ct]^b
18S	9	0.18	2.36	9	0.31	4.15	9	0.13	1.75	9	0.06	0.83
25S	9	0.57	5.80	9	0.46	4.68	9	0.21	2.24	9	0.23	2.36
ACT	9	0.23	1.24	9	0.37	1.93	9	0.24	1.22	9	0.18	0.91
β-TUB	9	0.21	0.75	9	0.34	1.21	9	0.35	1.23	9	0.46	1.6
eEF1-a	9	0.38	2.19	9	0.36	2.05	9	0.25	1.43	9	0.3	1.66
eIF-4a	9	0.32	1.53	9	0.34	1.63	9	0.31	1.48	9	0.32	1.51
UBC	9	0.14	0.63	9	0.17	0.77	9	0.22	0.99	9	0.22	0.97
UBQ5	9	0.37	2.08	9	0.43	2.37	9	0.19	1.04	9	0.22	1.17
UBQ10	9	0.42	2.30	9	0.43	2.31	9	0.23	1.23	9	0.25	1.29
GADPH	9	0.31	1.18	9	0.32	1.21	9	0.27	1.01	9	0.31	1.16

ISSN: 2661-8974