Skip to main content

Table 3 Gene expression stability of 10 candidate reference genes calculated by Bestkeeper

From: Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

Gene symbol Water Xoo MT-Water MT-Xoo
na SD (± Ct) CV [% Ct]b na SD (± Ct) CV [% Ct]b na SD (± Ct) CV [% Ct]b na SD (± Ct) CV [% Ct]b
18S 9 0.18 2.36 9 0.31 4.15 9 0.13 1.75 9 0.06 0.83
25S 9 0.57 5.80 9 0.46 4.68 9 0.21 2.24 9 0.23 2.36
ACT 9 0.23 1.24 9 0.37 1.93 9 0.24 1.22 9 0.18 0.91
β-TUB 9 0.21 0.75 9 0.34 1.21 9 0.35 1.23 9 0.46 1.6
eEF1-a 9 0.38 2.19 9 0.36 2.05 9 0.25 1.43 9 0.3 1.66
eIF-4a 9 0.32 1.53 9 0.34 1.63 9 0.31 1.48 9 0.32 1.51
UBC 9 0.14 0.63 9 0.17 0.77 9 0.22 0.99 9 0.22 0.97
UBQ5 9 0.37 2.08 9 0.43 2.37 9 0.19 1.04 9 0.22 1.17
UBQ10 9 0.42 2.30 9 0.43 2.31 9 0.23 1.23 9 0.25 1.29
GADPH 9 0.31 1.18 9 0.32 1.21 9 0.27 1.01 9 0.31 1.16
  1. aNumber of samples
  2. bCoefficient of variation expressed as the percentage of the Ct value