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Table 4 Ranking of the candidate reference genes according to their stability value calculated using geNorm, Normfinder and Bestkeeper in PX099-infected rice plant

From: Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

Sample Water Xoo
Genes BestKeeper gerNorm Normfinder BestKeeper gerNorm Normfinder
M value Rank
Order
M value Rank Order M value Rank Order M value Rank Order M value Rank Order M value Rank Order
18S 0.18 2 0.071812 1 0.205 2 0.31 2 0.085747 4 0.294 2
25S 0.57 10 0.209846 9 0.595 10 0.46 8 0.150497 7 0.591 10
ACT 0.23 4 0.106165 3 0.342 5 0.37 6 0.130881 6 0.462 8
β-TUB 0.21 3 0.081461 2 0.282 4 0.34 4 0.101257 5 0.353 3
eEF1-a 0.38 8 0.16297 7 0.346 6 0.36 5 0.063164 2 0.486 9
eIF-4a 0.32 6 0.143502 5 0.273 3 0.34 4 0.074115 3 0.354 4
UBC 0.14 1 0.071812 1 0.114 1 0.17 1 0.184964 9 0.111 1
UBQ5 0.37 7 0.156697 6 0.545 9 0.43 7 0.035446 1 0.455 7
UBQ10 0.42 9 0.17176 8 0.443 8 0.43 7 0.035446 1 0.439 6
GADPH 0.31 5 0.128316 4 0.348 7 0.32 3 0.16996 8 0.428 5