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Table 4 Ranking of the candidate reference genes according to their stability value calculated using geNorm, Normfinder and Bestkeeper in PX099-infected rice plant

From: Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

Sample

Water

Xoo

Genes

BestKeeper

gerNorm

Normfinder

BestKeeper

gerNorm

Normfinder

M value

Rank

Order

M value

Rank Order

M value

Rank Order

M value

Rank Order

M value

Rank Order

M value

Rank Order

18S

0.18

2

0.071812

1

0.205

2

0.31

2

0.085747

4

0.294

2

25S

0.57

10

0.209846

9

0.595

10

0.46

8

0.150497

7

0.591

10

ACT

0.23

4

0.106165

3

0.342

5

0.37

6

0.130881

6

0.462

8

β-TUB

0.21

3

0.081461

2

0.282

4

0.34

4

0.101257

5

0.353

3

eEF1-a

0.38

8

0.16297

7

0.346

6

0.36

5

0.063164

2

0.486

9

eIF-4a

0.32

6

0.143502

5

0.273

3

0.34

4

0.074115

3

0.354

4

UBC

0.14

1

0.071812

1

0.114

1

0.17

1

0.184964

9

0.111

1

UBQ5

0.37

7

0.156697

6

0.545

9

0.43

7

0.035446

1

0.455

7

UBQ10

0.42

9

0.17176

8

0.443

8

0.43

7

0.035446

1

0.439

6

GADPH

0.31

5

0.128316

4

0.348

7

0.32

3

0.16996

8

0.428

5

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Food Production, Processing and Nutrition

ISSN: 2661-8974

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