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Table 5 Ranking of the candidate reference genes according to their stability value calculated using geNorm, Normfinder and Bestkeeper in PX099-infected rice plant under MT treatments

From: Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

Sample

MT-Water

MT-Xoo

Genes

BestKeeper

gerNorm

Normfinder

BestKeeper

gerNorm

Normfinder

M value

Rank Order

M value

Rank Order

M value

Rank Order

M value

Rank Order

M value

Rank Order

M value

Rank Order

18S

0.13

1

0.112076

8

0.289

7

0.06

1

0.147335

8

0.205

7

25S

0.21

3

0.073051

4

0.442

10

0.23

4

0.179034

9

0.489

10

ACT

0.24

6

0.104181

7

0.307

9

0.18

2

0.103178

6

0.134

5

β-TUB

0.35

10

0.127072

9

0.3

8

0.46

9

0.076681

4

0.372

9

eEF1-a

0.25

7

0.051968

1

0.099

4

0.3

6

0.031131

1

0.103

3

eIF-4a

0.31

9

0.087268

5

0.176

5

0.32

8

0.031131

1

0.134

4

UBC

0.22

4

0.051968

1

0.074

2

0.22

3

0.064671

3

0.024

2

UBQ5

0.19

2

0.060874

3

0.063

1

0.22

3

0.053811

2

0.024

1

UBQ10

0.23

5

0.054308

2

0.085

3

0.25

5

0.086203

5

0.149

6

GADPH

0.27

8

0.095592

6

0.188

6

0.31

7

0.127123

7

0.26

8