Skip to main content

Table 5 Ranking of the candidate reference genes according to their stability value calculated using geNorm, Normfinder and Bestkeeper in PX099-infected rice plant under MT treatments

From: Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

Sample MT-Water MT-Xoo
Genes BestKeeper gerNorm Normfinder BestKeeper gerNorm Normfinder
M value Rank Order M value Rank Order M value Rank Order M value Rank Order M value Rank Order M value Rank Order
18S 0.13 1 0.112076 8 0.289 7 0.06 1 0.147335 8 0.205 7
25S 0.21 3 0.073051 4 0.442 10 0.23 4 0.179034 9 0.489 10
ACT 0.24 6 0.104181 7 0.307 9 0.18 2 0.103178 6 0.134 5
β-TUB 0.35 10 0.127072 9 0.3 8 0.46 9 0.076681 4 0.372 9
eEF1-a 0.25 7 0.051968 1 0.099 4 0.3 6 0.031131 1 0.103 3
eIF-4a 0.31 9 0.087268 5 0.176 5 0.32 8 0.031131 1 0.134 4
UBC 0.22 4 0.051968 1 0.074 2 0.22 3 0.064671 3 0.024 2
UBQ5 0.19 2 0.060874 3 0.063 1 0.22 3 0.053811 2 0.024 1
UBQ10 0.23 5 0.054308 2 0.085 3 0.25 5 0.086203 5 0.149 6
GADPH 0.27 8 0.095592 6 0.188 6 0.31 7 0.127123 7 0.26 8