Table 1 Molecular markers of SSIIa, SSIIIa and Wxmp genes used in this study
Genes | Primer sequences | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
SSIIa (L22) | F: CTTTGATAGTTCGAATGGTT | 251/248 | 55 |
R: CAATGTTTCTCCGTGATGAT | |||
SSIIIa (L25) | F: GAACTTGTGCCTTAAGCTGACTG | 197/177 | 55 |
R: GGAATAGTAAGCCGAAGGACTT | |||
Wxmp | O-F: 5′-ATGTTGTGTTCTTGTGTTCTTTGCAGGC3′ | 439/200 | 65 |
O-R: 5′-GTAGATCTTCTCACCGGTCTTTCCCCAA3′ | |||
I-F: 5′-GGGTGAGGTTTTTCCATTGCTACAATCG3′ | 439/292 | 65 | |
I-R: 5′-GTCGATGAACACACGGTCGACTCAAT3′ |