Fig. 4

Semi-quantification of Cld-4 and Cld-2 expression in ileum. Ileum was collected from D-mannitol group (n = 6). Cld-4 and Cld-2 expression were evaluated using 2^-ΔΔCt method. The mean Ct value of target genes in the experimental group was normalized to the Ct value of GAPDH to give a ΔCt value, which was further normalized to NC group to obtain ΔΔCt. Bars represented the 2^-ΔΔCt and RQ min/max values. RQ values are derived using standard deviation. Differences between groups were considered significantly different when p* < 0.05, **p < 0.01 and ***p < 0.001 relative to the LPS group