Fig. 5From: Potent antioxidant peptides derived from honey major protein enhance tolerance of eukaryotic cells toward oxidative stressFluorescent ex vivo imaging of ROS in HCT-116 cells oxidized with H2O2 or DEM. Cells (105 cells/L) were treated as in Fig. 4 and fixed with 2% paraformaldehyde in PBS and observed with Keyence BZ-9000 fluorescence microscope under antifade reagent. The non-fluorescent DCFH-DA is oxidized by ROS into DCF, which irradiates green/yellow fluorescence into and around the stressed cellsBack to article page