Fig. 4

CBSF suppressed the ROS level in db/db mouse aortae both in vivo and ex vivo. Representative confocal images (a) with summarized data (b) of DHE stain intensity in isolated aortic rings from db/m⁺ and db/db mice with and without in vivo CBSF treatment (25 mg/kg/day). Representative confocal images (c) and summarized data (d) of DHE stain intensity in isolated aortic rings from non-treated db/m⁺ and db/db mice with and without ex vivo CBSF incubation (0.4 μg/mL). Data are presented in means ± SEM; n = 5; *p < 0.05, **p < 0.01. CBSF, crocodile blood soluble fraction; ROS, reactive oxygen species; DHE, dihydroethidium