Skip to main content

Table 7 Tentative identification of main polyphenols in chromatographs of Moringa oleifera fresh and processed Leaf Extract

From: Impact of post harvest treatment on antioxidant activity and phenolic profile of Moringa oleifera lam leaves

No RT (min.) Mass (m/z) Diagnostic fragments Peak fragment Fresh Roasted Dried Fermented/Oxidised Blanched Identity
1 1.05   248; 325; 381; 448; 523; 723 381   
2 1.36 432 162; 180; 230; 253; 268; 270; 308;
414; 432; 468
Apigenin (270) + glucosyl (162) = 432
414, 432   Apigenin glucoside
3 3.18 355 163; 209; 227; 355; 379; 425; 726;
732
Caffeoyl(162) + quinic acid(192) = 354
163 Caffeoylquinic acid (chlorogenic acid)
4 3.78 339 147; 165; 227; 339; 419; 457; 499;
541; 595; 701
Coumaroyl (147)
147 Coumaroylquinic acid
5 4.03 355 147; 163; 177; 209; 341; 355; 369;
579
Caffeoyl(162) + quinic acid(192) = 354
163 Caffeoylquinic acid (chlorogenic acid)
6 4.32   163; 209; 227; 295; 322; 340; 457;
511; 557; 577; 595
595  
7 5.29 611 147; 163; 180; 303; 435; 449; 453;
465; 611
Quercetin (303) + rhamnosyl
(146) = 449
Quercetin(303) + glucosyl(162) = 465
+ rhamnosyl (146) = 611
303   Rutin
8 5.56 465 303; 465
Quercetin (303) + glucosyl(162) = 465
303 Isoquercetin (Querctin-3-O-glucoside)
9 5.83 551 303; 551; 635
Quercetin = 303 + Malonyl-glucosyl
(248) = 551
303 Quercetin-3-O-malonyl-glucoside
10 6.02 449 147; 180: 191; 196; 210; 230;
235; 249; 265; 270; 276; 287;
449; 551; 637; 679; 875
Kaempferol
(287) + glucosyl(162) = 449 + 230 = 679
287 Kaempferol-3-O-glucoside
11 6.20 (roasted) 507 180; 187; 205; 222; 303; 327; 345;
507; 529
Glucosyl(162) + acetyl(42) = 204 glucosyl (162) + Na (23) =185
glucose (180) + acetyl(42) = 222
quercetin(303) + acetyl(42) = 345 quercetin(303) + Na(23) = 326
Quercetin(303) + Glucosyl-acetyl(204)
=507 + Na(23) = 530
303       Quercetin-3-O-acetyl glucoside
(Na salt)
12 6.40 535 287; 535
Kaempferol(287);
Malonyl-glucoside = 248
535–287 = 248
287   Kaempferol-3-O-malonyl-glucoside
13 6.82 491 287; 491; 513
Kaempferol(287) +
Glucosyl-acetyl(204) = 491
287       Kaempferol-3-O-acetylglucoside
543 (Na salt)
14 10.72 593 593 593      Quercetin-3-O-rhamnoside
15    635; 778; 1269 635    
16           
17          
18           
19