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Table 2 Glucosinolates identified by LC-HRMS/MS.a

From: Effect of combined light-emitting diodes on the accumulation of glucosinolates in Brassica microgreens

#

Glucosinolate

Retention

time, min

Precursor

ion, m/z

Fragment ions, m/z

1

Glucoiberin

2.91

422.0257

96.9604, 95.9526, 74.9911, 79.9575, 195.0336, 358.0276, 259.0125

2

Progoitrin

3.32

388.0379

96.9604, 95.9526, 74.9911, 79.9575, 195.0336, 135.9713, 259.0129, 274.9904

3

Sinigrin

3.5

358.0272

96.9601, 74.9910, 79.9574, 195.0331, 161.9866, 116.0176, 259.0127, 274.9901

4

Glucoraphanin

3.77

436.0413

96.9604, 95.9526, 74.9911, 79.9575, 178.0183, 372.0430, 195.0331, 259.0133

5

Gluconapoleiferin

5.02

402.0534

96.9604, 95.9526, 74.9911, 79.9575, 195.0336,160.0443, 259.0115, 274.9905

6

Glucoalyssin

5.18

450.0569

96.9604, 95.9526, 74.9911, 79.9575, 192.0339, 195.0341, 386.0587, 259.0143, 274.9910

7

Gluconapin

5.51

372.0430

96.9604, 95.9526, 74.9911, 79.9575, 195.0336, 178.9847, 259.0128, 274.9903

8

4-Hydroxyglucobrassicin

6.37

463.0486

96.9604, 95.9526, 74.9911, 79.9575, 195.0334, 221.0391, 267.00812 285.0184

9

Glucoerucin

9.32

420.0462

96.9605, 74.9911, 178.0369, 195.0334, 259.01315, 274.9902, 226.9877

10

Glucobrassicin

10.33

447.0537

96.9605, 95.9527, 74.9911, 79.9576, 259.0128, 274.9902, 205.0445, 195.0336

11

Gluconasturtiin

13.1

422.0587

96.9604, 95.9526, 74.9911, 79.9575, 259.0127, 180.0490, 195.0335, 274.9902, 229.0001

12

4-Methoxyglucobrassicin

15.68

477.0643

96.9603, 95.9525, 74.9910, 79.9574, 259.0119, 274.9899, 235.0546, 195.0338, 119.0350

13

Neoglucobrassicin

19.82

477.0643

96.9605, 95.9527, 74.9912, 79.9574, 154.0543, 259.0127, 170.0490, 205.0443, 282.9848

  1. a Only one extraction replica was used for quantification, as the semi-targeted analysis revealed high fidelity (RSD < 5%) of extracted samples in all microgreens tested