Table 2 Box-Behnken design and results
| Â | Factor 1 | Factor 2 | Factor 3 | Response 1 |
|---|---|---|---|---|
Run | A: RAM concentration | B: Time | C: Water bath temperature (Reaction temperature) | SDG |
|  | (%) | (h) | (°C) | (mg/g) |
1 | 40 | 5 | 60 (56.0) | 18.98 |
2 | 30 | 4 | 60 (58.8) | 12.63 |
3 | 30 | 5 | 70 (66.7) | 19.84 |
4 | 30 | 5 | 70 (66.7) | 19.32 |
5 | 30 | 5 | 70 (66.7) | 20.50 |
6 | 20 | 6 | 70 (67.8) | 17.51 |
7 | 20 | 5 | 80 (76.0) | 20.32 |
8 | 20 | 4 | 70 (67.8) | 16.63 |
9 | 30 | 4 | 80 (75.4) | 20.95 |
10 | 40 | 6 | 70 (64.8) | 20.36 |
11 | 40 | 4 | 70 (64.8) | 16.15 |
12 | 30 | 6 | 80 (75.4) | 21.35 |
13 | 30 | 5 | 70 (66.7) | 20.71 |
14 | 30 | 5 | 70 (66.7) | 21.98 |
15 | 20 | 5 | 60 (58.9) | 18.24 |
16 | 30 | 6 | 60 (58.8) | 20.49 |
17 | 40 | 5 | 80 (75.2) | 22.83 |