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Table 3 Central composite design and response for the enzymatic synthesis of mono- and dioleyl p-coumarates

From: Mono- and dioleyl p-coumarate phenolipids and their antioxidant activity in a muscle food model system

Run

Pattern

Variables

Response (Y) degree of incorporation

X1 (wt%)

X2 (h)

X3 (ratio)

1c

0 0 0

10

216

9

2.85 ± 0.11

2

+ + +

15

288

16

6.43 ± 0.40

3

− + −

5

288

2

1.25 ± 0.12

4

0 0 a

10

216

2

1.66 ± 0.29

5c

0 0 0

10

216

9

3.32 ± 0.08

6

+ − −

15

144

2

0.09 ± 0.04

7

− + +

5

288

16

2.93 ± 0.75

8

+ + −

15

288

2

2.23 ± 0.13

9

0 0 A

10

216

16

4.40 ± 0.67

10

− − +

5

144

16

0.98 ± 0.01

11

A 0 0

15

216

9

4.00 ± 0.34

12

0 A 0

10

288

9

4.13 ± 0.34

13

+ − +

15

144

16

2.31 ± 0.32

14

0 a 0

10

144

9

1.46 ± 0.28

15c

0 0 0

10

216

9

2.87 ± 0.07

16

5

144

2

0.04 ± 0.01

17

a 0 0

5

216

9

1.87 ± 0.45

  1. Note: pattern identifies the coding of the factors. It shows all the codings with “+” for high, “-” for low factor, “a” and “A” for low and high axial values, and “0” for midrange; X1: enzyme load; X2: reaction time; X3: mole ratio of substrates; and c: centre points