Plant food anti-nutritional factors and their reduction strategies: an overview

Legumes and cereals contain high amounts of macronutrients and micronutrients but also anti-nutritional factors. Major anti-nutritional factors, which are found in edible crops include saponins, tannins, phytic acid, gossypol, lectins, protease inhibitors, amylase inhibitor, and goitrogens. Anti-nutritional factors combine with nutrients and act as the major concern because of reduced nutrient bioavailability. Various other factors like trypsin inhibitors and phytates, which are present mainly in legumes and cereals, reduce the digestibility of proteins and mineral absorption. Anti-nutrients are one of the key factors, which reduce the bioavailability of various components of the cereals and legumes. These factors can cause micronutrient malnutrition and mineral deficiencies. There are various traditional methods and technologies, which can be used to reduce the levels of these anti-nutrient factors. Several processing techniques and methods such as fermentation, germination, debranning, autoclaving, soaking etc. are used to reduce the anti-nutrient contents in foods. By using various methods alone or in combinations, it is possible to reduce the level of anti-nutrients in foods. This review is focused on different types of anti-nutrients, and possible processing methods that can be used to reduce the level of these factors in food products. A brief overview of beneficial effects of anti-nutrients and reduction strategy.


Introduction
In Asian countries, cereals and legumes are considered as major staple foods. Cereal grains such as rice, wheat and maize belong to the grass family Graminae and hold valuable place within the staple food crops, because they are consumed throughout the world. Cereal grains provide ample amounts of carbohydrates, proteins, vitamins and most importantly dietary fibers, which are necessary for our daily diet as well as growth and maintenance of the human body (Nadeem et al. 2010). Wheat is one of the key edible food crops, which is consumed by almost onethird of the world's population. Wheat is the most diverse crop, which is grown throughout the world with approximately 750 million tons produced annually (FAOSTAT 2016). Wheat is mainly considered a high nutritive value cereal crop because of its composition, and contents of macronutrients like proteins, carbohydrates and fats, in addition to minerals such as zinc, phosphorus, iron, calcium, magnesium (FAO 2018a, b). Recently, wheat-based foodstuffs have gained extensive attention in the consumer market and also from the food companies due to their health beneficial components. One example is the wheat flour-based cookies, which are made by baking into a product with very little moisture content and serve as source of energy for consumers (Adeyeye and Akingbala 2016;Okaka 1997). Maize or corn (Zea mays L.) is the key cereal crop, which is cultivated throughout the world and considered as a vital source of food for humans as well as feed for livestock (Tenaillon and Charcosset 2011;Gwirtz and Garcia-Casal 2014). After wheat and rice consumption levels, corn is considered as the 3 rd most important cereal crop worldwide (De Vasconcelos et al. 2013). Corn is consumed in various forms such as snacks, main dishes and children's foods; it is cultivated in large parts of the world mainly in Asia, Africa and America (Ekpa et al. 2018;Aoudou et al. 2012). Corn contains 65 to 84% starch, 9 to 10% protein, 3 to 5% fat, 3% ash and 2 to 3% fibre (Ihekoronye and Ngoddy 1985). Corn consists of ample amounts of essential minerals and the B vitamins; therefore, when consumed as whole grains, it could deliver sufficient quantity of nutrients (Ranum et al. 2014). Moreover, corn bran is a rich source of dietary fibre that can be applied to human foods, as well as in animal feed formulations (Rose et al. 2010). Corn possesses rich amounts of water and fat-soluble vitamins, which act as precursors for vitamin A (Oboh et al. 2010). In respect of nutritional quality, cereals contain low amounts of proteins but high amounts of carbohydrates. For example, wheat flour contains 10% protein content but nearly 87% of carbohydates, while Bajra or Pearl Millet (P. glaucum) contains 11 g of protein and approximately 73 g of carbohydrates (Kavitha and Parimalavalli 2014;Malik 2015). In terms of nutritional composition, cereals are rich sources of carbohydrates, vitamins and fibre but they are deficient (limiting) in the amino acid lysine. In contrast, legumes are rich sources of lysine; therefore, cereal flours can be supplemented with legumes to overcome the limiting amino acid problem (Katina et al. 2005;Awolu et al. 2015Awolu et al. , 2017. Cereal grains are also considered as rich sources of minerals mainly zinc and iron, in addition to ample amounts of vitamins and calories (Temba et al. 2016). Many cereal bars also come into consideration because some snack bars made from cereals, may be formulated to contain lesser amounts of total calories (Suhem et al. 2015). Cereals, which come under coarse category are also known as 'nutricereals' due to their exceptional functional and nutritional properties. Coarse cereals commonly include corn, sorghum, oats, barley, pearl millet and finger millet (Kaur et al. 2014). During growth, plants synthesize various types of phytochemicals or secondary metabolites by using shikimate pathways (Zhang et al. 2015). Along with corn and wheat, rice (Oryza sativa L.) is one of the principal edible cereal crops, which is consumed for human nutrition. After sugarcane (1.9 billion tonnes) and corn (1.0 billion tonnes), rice is the third highest cultivated staple crop worldwide at 741.5 million tonnes (Kennedy and Burlingame 2003;FAO-STAT 2017). In the Asian regular diet, cooked rice is mainly consumed as a whole grain and it adds about 40-80% to the over-all calorie intake (Paramita et al. 2002;Hossain et al. 2009;Singh et al. 2005). In terms of various advantages, rice starch is much better than corn with respect to flavor (bland), colour (white), greater resistance to acid and hypo-allergenic properties (Wani et al. 2012). Pearl millet is considered an edible crop, which shows resistance against drought conditions and also cultivated mainly in sub-saharan Africa and India as an essential food, though India is the main producing country (Wang et al. 2018).
In traditional systems, indigenous knowledge plays a key role in disease diagnosis and health care practices, which has encouraged consumption of cereals throughout the world as a main source of nutrients in various diets. In addition, recent trends indicate an increased demand for conventional or major tropical cereals. However, millets have been neglected and are underutilized even though they have tremendous potential as an economic and food crop. Food quality assessment of this type of underutilized crop can lead to increased sustainability or food security while improving the economic condition and human health of rural populations (Gopalan et al. 1989;Ebert 2014). This is because millets can be consumed as flour, breakfast food with milk, rolled into balls or parboiled and are recognized as high energy-containing foods, which can help in combating malnutrition (FAO 2018a(FAO , b, 2009). Consumption of whole cereal grains could protect against obesity, cardiovascular diseases, diabetes and other types of chronic health disorders (Oghbaei and Prakash 2016). For example, millets have antioxidative properties due to the presence of phenolic compounds, which have been shown to protect against cardiovascular diseases (Kumari et al. 2019).
Legumes are edible crops that belong to the Leguminosae family and second only to cereals in terms of level of human consumption (Sánchez-Chino et al. 2015;Seigler 2005). Common legumes, which are consumed all over the world include cranberry beans, black beans, Great Northern beans, navy beans, chickpeas, kidney beans, lentils etc. Legume grains contain desirable levels of ingredients that can enhance nutritional quality such as high protein concentration, potassium, fiber, and low glycemic index. Consumption of legume seeds is believed to have a strong impact on blood pressure reduction while conferring antioxidant benefits (Polak et al. 2015;Vaz Patto et al. 2015). Soybean (Glycine max) comes under the leguminous family; it is widely grown in tropical, subtropical and temperate climates, containing a higher amount of protein (36%) than cereals, nearly 30% of carbohydrates and exceptional amounts of minerals, vitamins and dietary fiber. Soybean is one of the most important crops used for producing edible oil because the seed contains about 20% of oil (Edema et al. 2005; Food and Agricultural Organization of the United Nations 2004; Gibson and Benson 2005). Soybean could also be used for making nutritious food products such as cookies, snack foods, bread, soups and pasta due to the rich protein concentration as well as balanced amino acid profile (Edema et al. 2005). Silva et al. (2019) reported that soybean is also used for producing soymilk, which can be consumed as such or used for preparing fermented soymilk, tofu and soy yogurt.
It has been found that significant amounts of sulphurcontaining amino acids like methionine and cysteine are present in cereal grains. Methionine and cysteine have many nutraceutical properties that are helpful to prevent health disorders such as lowering the risk of cardiovascular diseases, reduced blood pressure, and decreased incidence of tumor. Leguminous crops mainly provide ample amounts of protein when compared to cereals. In comparison to meat, eggs and poultry, soybean is the only crop, which provides a cheap and high quality protein (Edema et al. 2005; Food and Agricultural Organization of the United Nations 2004). In developing countries, one of the key nutritional problems is protein energy malnutrition (Edema et al. 2005). Soybean has much supreme concentration of protein, fibre, minerals and vitamins; the high concentration of fibre in soybeans may help in reducing the incidence of type-2 diabetes, constipation and hypercholesterolemia (Gibson and Benson 2005;Edema et al. 2005; Food and Agricultural Organization of the United Nations 2004). Peanut or groundnut (Arachis hypogaea L.) also comes under the leguminous family; it is a seed crop, which is cultivated in tropical and subtropical climates. It consumed in different forms including as a component of formulated or processed foods, roasted nut and also for edible oil extraction (Stalker 1997). Groundnut seeds are good sources of several essential minerals such as calcium, phosphorus, magnesium and zinc as well as vitamin B1 (FAO 2002). Groundnut also contains ample amounts of vitamin E (αtocopherol), which is widely used to prevent oxidative deterioration in lipid-rich foods (de Camargo and Silva et al. (2019). Kersting's groundnut (Kerstigiella geocarpa Harms) is considered an underutilized legume; it consists of high amounts of essential minerals, protein and amino acids. The crop is grown mainly in Africa and is considered an alternative to high protein foods (Bayorbor et al. 2010).
In rural areas, micronutrient deficiencies such as those involving vitamins and minerals are one of the biggest causes of health-related problems (Black et al. 2013). This is because the presence of micronutrients in bulk amounts is not the only important nutritional factor but also their bioavailability is critical in meeting human nutrient needs. In raw foodstuffs, the lower mineral bioavailability decreases the nutritional value of these foods, which could lead to the development of metabolic health disorders. Thus, the nutritional condition of a population can be improved by enhancing bioavailability of food nutrients (Bouis et al. 2019;Gupta et al. 2015). Unfortunately, millets, though cheap and can be grown easily are reported to have low a nutritional value due to the presence of anti-nutritional factors, which reduce nutrient bioavailability (Sarita and Singh 2016). Legumes also comprise of many natural toxicants or anti-nutrients, which include tannins, metal chelators, protease inhibitors, saponins, cyanogens, phytic acid, isoflavonoids, etc. (Pariza 1996). One of the major anti-nutrients is phytate, which chelates and mainly affects bioavailability of calcium and other micronutrients such as iron, copper and zinc. Some other factors such as polyphenols and oxalates are also considered as anti-nutrients that can limit food mineral bioavailability (Kaushik et al. 2018). However, phytochemicals such as phenolic compounds show antioxidant activity through their potency in scavenging ROS, reducing power and/or metal-chelating activity towards ferric and ferrous ions. While not all polyphenols exhibit chelating properties, phytochemicals like isoflavones, genistein and biochanin A are considered as ideal antioxidants because they possess the quality of reducing agents in addition to metal ionchelating properties .
Like other grains, several anti-nutritional factors are found in wheat, which may have effect on the digestibility of wheat products and by this means, affect human health (FAO 2018a, b). Anti-nutrients can have significant adverse effects on the nutritional value of foods; therefore, reducing their concentration in foods is a major goal in human nutrition. Subsequently, legumes are commonly eaten as protein sources along with cereals, but in order to eliminate the anti-nutrients, suitable processing of these food substances should be encouraged before their consumption (Reddy and Pierson 1994). Like other legumes, peanuts are also comprised of anti-nutrients, which can impair nutrient bioavailability through formation of indigestible complexes with minerals and proteins (Francis et al. 2002;Lönnerdal 2002). In addition to decreasing nutrient bioavailability, anti-nutrients can become toxic when present beyond a certain amount. Therefore, reduction in the levels of anti-nutritional factors in edible crops is an area of research interest due to the need to prevent toxicity and associated health problems caused by these anti-nutrients (Gemede and Ratta 2014).
The anti-nutritional factors, which reduce the nutritional value of foods can be reduced by the use of traditional food preparation methods such as fermentation, cooking, soaking and puffing. These food processing techniques reduce anti-nutritional factors, increase protein digestibility and improve the biological value of cereal crops (Handa et al. 2017;Jaybhaye and Srivastav 2015). Therefore, the main focus of this review is to discuss various anti-nutrients present in foods and also assess processing methods that can be used to reduce the concentration of anti-nutritional factors such as phytate, saponins, polyphenols and protease inhibitors.

Anti-nutrient factors
Several types of anti-nutritional factors with toxic potential have been measured in foods and shown to be heatstable or heat-labile. These factors include saponins, tannins, phytic acid, gossypol, lectins, protease inhibitors, amylase inhibitors, antivitamin factors, metal binding ingredients, goitrogens, etc. Nutrition-related problems and harmful effects to human health are raised by these factors, which are present in the seeds of cereals and legumes. Figure 1 shows a brief overview of the adverse effects of key anti-nutrients that are present in foods.

Saponins
Saponins are commonly considered as non-volatile, surface active secondary metabolites, which are broadly dispersed in nature but found principally in plants. Saponins are steroids or triterpenes and contain a sugar moiety in their structure. They are naturally produced as foam-producing triterpene or glycosides by many plant species, including groundnut, lupin, oil seeds, etc. (Kiranmayi 2014). Triterpenoid saponins are usually found in most cultivated crops such as legumes (e.g. soybean, peanuts, chickpeas, broad beans, and lentils), sunflower seeds, spinach leaves, tea leaves, quinoa seeds, sugar beet and allium species. In contrast, steroid saponins are generally present in food plants such as oats, yucca, tomato seeds, fenugreek seeds, asparagus, aubergine, and yam (Fenwick et al. 1991;Moses et al. 2014). Saponins are plant-derived secondary compounds, which are found in more than 100 families of wild and cultivated plants that belong to the Magnoliophyta division. Magnoliophyta can be divided into two key classes: Liliopsida and Magnoliopsida, which contain majority of species that produce saponins (Vincken et al. 2007). Saponins have a property of being able to interact with the cholesterol group of erythrocyte membranes, which leads to hemolysis (Fleck et al. 2019). Previous studies have reported that saponins also showed inhibitory activities of digestive enzymes such as amylase, glucosidase, trypsin, chymotrypsin and lipase, which can cause indigestion-related health disorders (Ali et al. 2006;Birari and Bhutani 2007;Ercan and El 2016;Lee et al. 2015;Liener 2003). Glucosidases are carbohydrate-hydrolyzing enzymes, which are mainly involved in the breakdown of glycosidic bonds in complex sugars. α-Glucosidase is one of the important glucosidases, which is present in the brush border of the small intestine. α-Glucosidase facilitates glucose absorption by breaking the glycosidic bonds in disaccharides and starch to produce the simpler and more absorbable monosaccharides (Kumar et al. 2011). Saponins are not readily hydrolyzed by the human digestive enzymes, therefore gastrointestinal digestion can be severely impaired (Amin et al. 2011). Previous studies have demonstrated that animal metabolism and health could be affected by saponins in different ways. The effects include bloating in ruminants, reduced nutrient absorption, decreased liver cholesterol and overall growth rate, and reduced intestinal absorption of many nutrients through binding of saponins to the small intestine cells (Addisu and Assefa 2016;Kregiel et al. 2017). It should be noted that the low levels of saponins in legumes may not be injurious to health but could become toxic when consumed at higher concentrations in the diet (Jansman et al. 1998). For example, sheep were died when fed saponin levels ≥ 150 mg/kg body weight (Williams 1978). Saponins are also considered as factors that reduce absorption of vitamins. It has been suggested that saponins can form complexes with various sterols that have similar structures as fat-soluble vitamins, which would interfere with sterol activity and absorption (Cheeke 1971). A previous study by Jenkins and Atwal (1994) reported that a diet prepared by using Gypsophila and Quillaja, which contained triterpenoid saponins, reduced the absorption of vitamins A and E when fed to chicks.

Phytates
Phytates or phytic acids occur naturally in the plant kingdom. Phytate is generally known as myo-inositol-1,2, 3,4,5,6-hexakis dihydrogen phosphate, which is present in foods at various levels ranging from 0.1 to 6.0% (Gupta et al. 2015). Phytic acid is a secondary compound, which concentrates naturally in plant seeds, mainly in legumes, peanuts, cereals, and oilseeds and generally found in all plant-based foods (Lolas 1976;García-Estepa et al. 1999). In several cases, phytates contain about 50 to 80% of the total phosphorous in seeds  (Lott et al. 2000;Raboy 2000). Because plant-based foods contain more amount of phytic acids than animal-based foods, the vegetarian diets culture in developing countries contribute to high ingestion levels (Kwun and Kwon 2000;Amirabdollahian and Ash 2010). According to a previous report, phytic acid hinders the activity of enzymes, which are necessary for protein degradation in the small intestine and stomach (Kies et al. 2006). Generally, phytic acids affect the bioavailability of minerals and has a strong effect on infants, pregnant and lactating women when large portions of cereal-based foods are consumed (Chan et al. 2007;Al Hasan et al. 2016). During germination of seeds, some native enzymes are activated, which degrade the phytic acid (Kaukovirta-Norja et al. 2004;Larsson and Sandberg 1992). In wheat and rice, which are generally recognized as monocotyledon crops, phytates are present in the bran or aleurone layer and can be easily separated during milling. On the other hand, in diacotyledons such as legumes, oilseeds and nuts, phytates are found in close association with proteins, which reduces ease of separation by a simple processing method like milling (Sinha and Khare 2017). Phytic acid is generally a negatively-charged structure, which generally binds with positively-charged metal ions such as zinc, iron, magnesium and calcium to make complexes and reduce the bioavailability of these ions through lower absorption rates. Mainly due to this chelating property, phytic acid is considered as a most effective anti-nutrient in foods, and a cause of mineral ions deficiencies in animal and human nutrition (Grases et al. 2017;Bora 2014).

Tannins
Tannins are phenolic compounds, which consist of molecular weights greater than 500 Da. One of the properties of these compounds is that they can precipitate proteins. Tannins are secondary compounds, which are formed in plant leaves, fruits and bark (Timotheo and Lauer 2018). Tannins usually affect protein digestibility and lead to reduction of essential amino acids by forming reversible and irreversible tannin-protein complexes between the hydroxyl group of tannins and the carbonyl group of proteins (Lampart-Szczapa et al. 2003;Raes et al. 2014). Proteins, which generally form complexes with tannins are relatively large and hydrophobic in nature, in addition to an open and flexible structure that is enriched with proline (Frutos et al. 2004). Tannins are largely concentrated in beverages, pomegranate, berry fruits and cocoa bean, although they are also found in cereals such as sorghum and barley (Serrano et al. 2009;Morzelle et al. 2019). In nature, there are two types of tannin groups: hydrolyzable (e.g. gallotannins and ellagitannins) and condensed (e.g. proanthocyanidins). Peanut and millets also contain proanthocyanidins (condensed tannins) in ample amounts (de Camargo and da Silva Lima 2019; Chandrasekara and Shahidi 2011). In ruminants, hydrolysable type of tannins are readily broken down during the digestion process. The breakdown products constitute a large amount of compounds, which can be toxic (Kumar 1992). Leguminous forage and some seeds generally contain mostly condensed tannins. Previous studies showed that goats are resistant to these tannins while cattle and sheep are sensitive (Smeriglio et al. 2017;Bhattarai et al. 2016;D'Mello 2000). Tannins accumulate mainly in the bran section of the legumes. When ingested, tannins form complexes with proteins, which cause inactivation of many digestive enzymes and decrease protein digestibility (Joye 2019).

Enzyme inhibitors
Proteinases are enzymes, which have diverse roles in improving nutritional and functional properties of various protein molecules (Salas et al. 2018). Various proteolytic actions are performed by proteases inhibitors (PIs) such as signal initiation, transmission and cellular apoptosis, inflammatory response, blood coagulation and several pathways of hormone processing (Gomes et al. 2011). Cereal seeds mainly contain plant serpins, one of the largest protease inhibitor family, which are also known as "suicide inhibitors" having molecular weight of 39 to 43 kDa and also found in several other species in the plant kingdom (Habib and Fazili 2007;Haq et al. 2005). Serpins are the effective inhibitors, which generally inhibit trypsin and chymotrypsin activities by acting upon the overlapping reactive sites of the enzymes (Dahl et al. 1996). Protease inhibitors are natural plant inhibitors, which have become an important research area due to their effective way of limiting enzyme activity by forming protein-protein interactions. They inhibit the enzyme activity through the catalytic mode by blocking the active site of the enzymes. The N-or C-terminus and the exposed loop of protease inhibitors are frequently considered important structural features for the inhibition of enzyme activity (Otlewski et al. 2005). Ragi contains bifunctional inhibitor, which inactivates the protein and starch-degrading enzymes by making trimetric complex interactions with trypsin and α-amylase, respectively (Shivaraj and Pattabiraman 1981). Legumes contain high amounts of protease inhibitors, α-amylase inhibitors and lectins, which could lead to low minerals bioavailability as well as reduced nutrients absorption and digestibility (Bajpai et al. 2005;Yasmin et al. 2008). Compared to legumes, cereals contains much lower amounts of these digestion inhibitors, especially those that act against proteases and amylases (Nikmaram et al. 2017). Within the gastrointestinal (GI) tract of animals, proteolytic enzyme activity could be severely inhibited by protease inhibitors present in diets (Nørgaard et al. 2019). The Kunitz trypsin inhibitor and Bowman-Birk inhibitor are two kinds of protease inhibitors, which are commonly found in soybean but are not readily inactivated by heat treatment due to the presence of disulfide bridges (Liu 1997;Van Der Ven et al. 2005). There are several enzyme inhibitors that are present in plant-derived foods but those that affect trypsin and α-amylase activities are the two key types, which are found in almost all cereals and legume-based foods. α-amylase mainly regulates the breakdown of carbohydrates such as polysaccharides into oligosaccharides. Therefore, enzyme inhibitors that specifically inhibit α-amylase activity will increase carbohydrate absorption time by delaying carbohydrate digestion. Due to the increased carbohydrate digestion time, glucose absorption rate is decreased and this affects the normal postprandial plasma glucose level (Bhutkar and Bhise 2012). Various previous studies have shown that seeds, which are rich in trypsin inhibitors may increase the satietogenic hormone i.e. cholecystokinin (CCK) and cause reduction in food intake and body weight (Serquiz et al. 2016;Chen et al. 2012;Ribeiro et al. 2015). Occurrence of trypsin inhibitors in human diets can lead to decreased growth rate by reducing protein digestion and availability of amino acids in addition to causing pancreatic hyperplasia (Adeyemo and Onilude 2013). However, several studies have reported that enzyme inhibition (e.g. alpha-amylase, alpha-glucosidase, lipase) may also provide health benefits related to the prevention of type 2 diabetes and obesity as discussed by Li and Tsao (2019).

Lectins and haemagglutinins
Lectins and hemagglutinins are a form of sugar-binding proteins, which easily attach to red blood cells to cause agglutination. These anti-nutrients are mainly found in foods, which are consumed in raw forms (Hamid et al. 2013). Cereals and legumes generally contain lectins, which are glycoproteins. In addition, transport and hydrolytic functions of the enterocyte would be impaired by consumption of foods that contain lectins (Krupa 2008). Phytohemagglutinin is a tetrameric glycoprotein with a molecular mass of 120 kDa, which is found in kidney beans and also consists of two diverse subunits (Lajolo and Genovese 2002). In rats, kidney bean phytohemagglutinin appears to upregulate the function and metabolism of the whole gastrointestinal tract, which includes growth of the small intestine, increased length of the tissue and number of intestinal crypt cells (Bardocz et al. 1995). In another study, phytohemagglutinins enhanced growth of the rat pancreas by increasing CCK hormone release; however, an independent mechanism was responsible for intestinal growth (Herzig et al. 1997). Purified lectins from beans or soybeans impaired rat growth, induced enlargement of the small intestine, caused damage to the epithelium of the small intestine, and stimulated hypertrophy and hyperplasia of the pancreas. Lectins impair nutrient absorption by binding to intestinal epithelial cells, and also cause damages in the intestinal tract, which allow bacterial population to come into contact with the blood stream (Muramoto 2017).

Strategies used to reduce food levels of antinutrients
Previous studies have shown that anti-nutrients cause adverse effects on diet value by reducing nutritional significance of foods. Prasad et al. (1963) reported that Egyptian boys were found deficient with zinc minerals, especially those that consumed bread and beans consistently. It is now very well accepted that phytate present in foods is one of the key concerns for the zinc deficiency. Jenkins and Atwal (1994) reported that dietary saponins reduced the growth and feed proficiency in chicks, while the absorption of vitamins A and E as well as lipids was also negatively impacted. A previous study by Lee et al. (1993) concluded that the metabolism of calcium, zinc and phosphorous was adversely affected when phytate was given to female rats. Besides their property of reduction of various minerals and nutrients, these anti-nutrients could cause toxicity when present in higher amounts in the diet. Due to these reasons, reduction in the anti-nutritional content of foods is of great interest. Different traditional methods and technological processing ways such as soaking, milling, debranning, roasting, cooking, germination and fermentation have been used for reducing these anti-nutritional components in foods. Here we describe various processing methods, which are used to decrease the concentration of phytate, tannin, saponins, etc. in foods (Table 1).

Milling
Milling is the most traditional method to separate the bran layer from the grains. It is a process by which grains are ground into flour. The milling technique removes anti-nutrients (e.g. phytic acid, lectins, tannins), which are present in the bran of grains, but this technique has a main disadvantage that it also removes important minerals (Gupta et al. 2015). For example, research on millet milling reported that the chemical composition of pearl millets was changed due to milling process. On the other hand, no much change was observed in pearl millet flour when processed through baking. However, milling and heating process during making of chapatti reduced the phytic acid and polyphenol contents in addition to significant improvements in starch and protein digestion (Chowdhury and Punia 1997). In another research, two varieties of pearl millets were used for evaluating their nutrients, anti-nutrients, and mineral bioavailability after milling them into whole flour, bran rich segment and semi-refined flour. The results of nutrient composition showed that no difference was found in semi-refined flour and whole flour except fat content, which was 1.3%. However, the contents of phytate and oxalate were found to be low in semi-refined flour when compared with whole flour, due to removal of the bran fraction (Suma and Urooj 2014).

Soaking
Soaking is an attractive method for removing antinutrient content of foods because it also reduces cooking time. Soaking also enhances release of enzymes (e.g. endogenous phytases), which are present in plant foods like almonds and other nuts and grains. Soaking generally provides essential moist conditions in nuts, grains and other edible seeds, which are required for their germination and associated reductions in level of enzyme inhibitors as well as other anti-nutrients to enhance digestibility and nutritional value (Kumari 2018). Soaking is also commonly required for fermentation, which can also be used to reduce the level of various anti-nutrients in foods (Gupta et al. 2015). Many of the anti-nutrients are water soluble in nature, which enhance their removal from foods through leaching. Soaking generally increases the hydration level of legumes and cereals, which make them soft and also activate an endogenous enzyme like phytase to enhance ease of further processing such as cooking or heating. A previous study stated that 6 h soaking reduced 27.9% and 24 h of soaking reduced 36.0% of phytic acid at room temperature in Mucana flagellipes (Udensi et al. 2008). Due to soaking, activity of phytase increased, which reduced the phytate component present in the grains. As a result of soaking and fermentation, phytochemicals are reduced due to leaching of water-soluble vitamins and minerals in grains and legumes (Ogbonna et al. 2012;Kruger et al. 2014). However, soaking commonly reduces the content of anti-nutrient phytochemicals like phytate, tannins, etc. Therefore, due to these benefits, it was recommended that wheat and barley should be consumed after soaking for a period of time (Gupta et al. 2015), especially 12 to 24 h (Ertas and Turker 2014); Mahgoub and Elhag 1998;Onwuka 2006). For example, during soaking, endogenous or exogenous phytase enzymes could enhance the in vitro solubility of minerals such as zinc and iron by 2 to 23% (Vashishth et al. 2017). A previous research carried out by Greiner and Konietzny (2006) showed that soaking reduced phytate content significantly at 45°C and 65°C. Soaking of grains and beans was found much effective to enhance the minerals concentration and protein availability, accompanied by reductions in phytic acid level (Coulibaly et al. 2011). Another study reported that phytic acid concentration in chickpea was deceased by 47.45 to 55.71% when the soaking time was increased from 2 to 12 h (Ertaş and Türker 2014).

Autoclave and cooking
Autoclave is an application, which is generally used for heat treatments. When this application is used on cereals and other plant-based foods, it activates the phytase enzyme as well as increases acidity (Ertop and Bektaş 2018). Most of the foods showed health benefits when consumed after autoclaving. For example, boiling of food grains reduced anti-nutrients content, which improved their nutritional value (Rehman and Shah 2005). Soaking and cooking also greatly decreased the phytic content in legume grains (Vadivel and Biesalski 2012). Food legumes are generally cooked by boiling or by using a pressure cooker prior to consumption. Previous studies also reported that boiling or cooking highly improved the nutritional value of foods by reducing their antinutritional (e.g. tannins and trypsin inhibitors) contents (Patterson et al. 2017). Another study by Vadivel and Biesalski (2012) reported that phytic acid concentration drastically decreased in legume grains when they were treated with cooking and soaking. A previous study by Vidal-Valverde et al. (1994) showed much reduced amounts of phytic acid in lentils when the seeds were treated with soaking followed by cooking application for a short period. Another research by Mustafa and Adem (2014) reported that whole wheat bread, which was treated through autoclave and microwave application showed reduced level of phytic acid, but the level of total free (unbound) mineral content was increased. This is because phytic acid possess mineral-chelating property; therefore, reduction in phytates lowers the level of bound minerals while enhancing free mineral contents. Another study also reported that the nutritional quality of legumes was much improved after cooking due to reductions in the contents of lectins and saponins (Maphosa and Jideani 2017). In another study, it was reported that anti-nutrients of black grams and mung beans were reduced by pressure-cooking when compared to normal cooking treatment (Kataria et al. 1989). A study by Shah (2001) observed that pressure-cooking reduced the tannins content, which led to improved black gram protein digestibility. Savage and Mårtensson (2010) also reported that oxalate content of taro leaves was reduced by 47% when boiled in water for 40 min, even though there was no significant reduction observed in oxalate content after baking for 40 min at 180°C. Roasting method also decreased the trypsin inhibitor activity significantly in soybean meal (Vagadia et al. 2017). Another study observed that several anti-nutritional factors were reduced significantly after autoclaving, soaking and cooking of legumes (Torres et al. 2016). Most of the previous studies concluded that autoclaving is the best method to reduce levels of several anti-nutritional compounds when compared to other processing methods (Shimelis and Rakshit 2007;Vadivel et al. 2008;Doss et al. 2011).

Germination
Germination is also considered as a highly suitable method for reducing the anti-nutrient components of plant-based foods (Nkhata et al. 2018). Germination of seeds generally activates the enzyme phytase, which degrades phytate and leads to decreased phytic acid concentration in the samples. Germination commonly changes the nutritional level, biochemical property and physical features of the foods. For reduction of cereals anti-nutritional content, this method is most frequently used (Laxmi et al. 2015;Oghbaei and Prakash 2016;Onyango et al. 2013). Germinated cereals showed enhanced activity of phytase-degrading enzyme while in non-germinated cereals the endogenous activity of phytase enzyme was observed in diminished amounts (Vashishth et al. 2017). After malting of millet samples for 72 h and 96 h, it was found that phytic acid content was reduced 23.95 and 45.3%, respectively (Makokha et al. 2002;Coulibaly et al. 2011). In a previous work by Azeke et al. (2011), it was observed that phytate content of cereal grains samples was reduced significantly when estimated after 10 days of germination. Another study conducted by Zhang et al. (2015) reported that antinutrient factors including total phenolic, flavonoid and tannin contents were increased in germinated buckwheat samples. Latest studies reported that germination also changes the isoflavone profile of soybean due to activation of β-glucosidases; this is important in enhancing nutritional value because isoflavones exhibit chelating properties (Yoshiara et al. 2018;). Reduction of anti-nutrients like tannin and phytic acid in germinated cereals increase the bioavailability of several minerals, which led to increased nutritional value of the food products (Ogbonna et al. 2012;Oghbaei and Prakash 2016). Another latest study by Singh et al. (2017) reported that when millets were processed by germination, maximum reductions in polyphenol contents (up to 75%) were found when compared to soaking, microwave treatment and fermentation.

Fermentation
Fermentation may be a useful strategy for reducing bacterial contamination of foods. For treating diarrhea in young children, fermented millet products are recommended as probiotics (Manisseri and Gudipati 2012;Nduti et al. 2016). Fermentation is a metabolic process in which sugars are oxidized to produce energy; it also improves the absorption of minerals from the plantbased foods. Fermentation is one of the processing methods, which is used in Africa to made cereals crops edible and also increase the nutritional quality as well as safety aspects of these foods, because cereals are not easily consumed in natural/raw forms (Galati et al. 2014).
In cereals, phytic acid normally forms complexes with the metal cations including iron, zinc, calcium and proteins. These complexes are generally degraded by enzymes, which require an optimum pH maintained by fermentation. Thus, this kind of degradation decreases the phytic acid content and liberates soluble iron, zinc and calcium, which enhance the nutritional level of food grains (Gibson et al. 2010). Fermentation of cereals by lactic acid bacteria (LAB) has been reported to increase free amino acids and their derivatives by proteolysis and by metabolic synthesis. Fermentation has been shown to improve the nutritional value of grains by increasing the content of essential amino acids such as lysine, methionine and tryptophan (Mohapatra et al. 2019). Previous reports observed that several anti-nutrients including protease inhibitors, phytic acids and tannins were reduced due to millet grain fermentation for 12 and 24 h (Coulibaly et al. 2011). Fermentation is such an important process, which significantly lowers the content of anti-nutrients such as phytic acid, tannins, and polyphenols of cereals (Simwaka et al. 2017). Fermentation also provides optimum pH conditions for enzymatic degradation of phytate, which is present in cereals in the form of complexes with polyvalent cations such as iron, zinc, calcium, magnesium and proteins. Such a reduction in phytate may increase the amount of soluble iron, zinc, calcium several folds (Gupta et al. 2015). Tannin levels may be reduced as a result of lactic acid fermentation, leading to increased absorption of iron, except in some high tannin cereals, where little or no improvement in iron availability has been reported (Ray and Didier 2014). Reduction in polyphenols may be because of the presence of phenolic oxidase during germination (Tian et al. 2019;Tajoddin et al. 2014). In most of the cereals, Lactobacillus spp. plays a major role in fermentation (Bhatia 2016). Lactobacillus spp. and Streptococcus spp.
are not very suitable bacteria for rice fermentation because they lack amylase, which is necessary for starch saccharification (Ray et al. 2016). In cases when cereal grains are used as natural medium for lactic acid fermentation, amylase needs to be added before or during fermentation or amylolytic bifido bacteria need to be used because these bacteria contain enough amylase, which is necessary for saccharification of the grain starch (Kim et al. 2000). A study reported that when germinated millets sprouts were fermented at 30°C with mixtures of probiotics culture consisting of Saccharomyces diasticus, Saccharomyces cerevisiae, Lactobacillus brevis and Lactobacillus fermentum for 72 h, approx. 88.3% reduction of phytic acid content was observed (Khetrapal and Chauhan 1990). Another study by Ragon et al. (2008) concluded that phytic acid (IP6) was reduced into lower forms, such as IP5, IP4, IP3, IP2, IP1 and myoinositol by the action of microbial enzymes when rice flour was subjected to natural fermentation. In a latest study, maize flour was fermented with a consortium of lactic acid bacteria by standard method with 12 h intervals to check the effect of fermentation on anti-nutritional factors (Ogodo et al. 2019). The results showed that with increasing fermentation period, significant (p < 0.05) reductions in anti-nutrients, including tannin, polyphenol, phytate and trypsin inhibitor activity were observed in the fermented maize. Results concluded that the anti-nutritional contents were reduced more in LAB-Consortium fermentation compared to spontaneous fermentation (Ogodo et al. 2019). Research was also conducted by Etsuyankpa et al. (2015) to evaluate the effect of microbial fermentation on antinutritional composition of local cassava products. Results of the study emphasized that fermentation by microorganisms significantly decreased (P < 0.05) the level of cyanide, tannins, phytate, oxalate and saponins by 86, 73, 72, 61, and 92%, respectively in the cassava products. A study by Samia et al. (2005) reported that fermentation and germination could enhance the nutritional level of cereals and legumes by altering the chemical composition and reduce the level of anti-nutritional factors.

Conclusions
This review provides essential information on the antinutritional components of cereal and legume seeds. The most common anti-nutrients present in plant materials include saponins, tannins, phytate, polyphenolic compounds, and protease inhibitors. These components interfere with the nutritional value of foods by reducing mineral absorption, protein digestibility and causing toxicity and health disorders when present in high concentrations. Nowadays, several strategies are used to overcome the effects of these food anti-nutrients, which include processing treatments such as milling, soaking, germination, autoclave and microwave treatment and fermentation. Various previous studies confirmed that fermentation is one of the best methods to reduce the anti-nutritional factors in foods when compared to all other methods. However, germination followed by fermentation also showed good results for reducing the level of anti-nutrients in foods. Microbial fermentation activates many endogenous enzymes like phytase, which generally degrades phytate in the food; phytate is one of the largest anti-nutrients, which is present in food crops. Previous works have established that anti-nutrients have close negative relationship with the micronutrient bioavailability because higher contents of anti-nutrients reduce availability or absorption of minerals and could lead to nutrients deficiency or malnutrition. Therefore, quality of food crops like cereals and grains can be improved by subjecting them to various processing methods, especially germination and fermentation.