Production and evaluation of novel functional extruded corn snacks fortified with ginger, bay leaves and turmeric powder

Extruded corn snacks are accepted by all human ages especially children, but they have low functional value. Therefore, corn extruded snacks contain rich nutraceuticals dried herbs including Laurus nobilis (T1), Curcuma longa (T2), Zingiber officinale Roscoe (T3), and the mixture of these herbs (T4) were manufactured and analyzed. The results declared that all the herbal extruded corn snacks had significantly higher ash, fibers, minerals, and vitamins A and B6. For minerals, the highest percent of increase compared to control was achieved by Fe, K, Ca, Zn content in order, being the highest in T4. The contents of Vitamin A and B6 were ranged from 283 to 445 IU/100 g and from 0.01 to 0.08 mg/100 g for the herbal extrudates, respectively. The increased percent in herbal corn snacks relative to control ranged from 743 to 452%, 188 to 17.6%, and from 313 to 99% for total phenolics, flavonoids, and antioxidant activity. Besides, the highest number of phenolic compounds was recorded in T4. Despite the fact that approximately all herbal extruded products had good texture and color characteristics, the best formulation was T2 and T4 corn snacks. Furthermore, the extruded products were microbiologically safe for up to 9 months. The formulation of herbal-corn snacks could fulfill consumers’ requirement for ready-to-eat-healthy foods with acceptable sensory attributes and also economically suitable for the food industry.


Introduction
The progress in food products has provided information about the urgent needs of nutrients for humans. Food manufacturers are creating innovative food products that form part of the next generation of food that not only are ready for immediate use, but also have a special nutritional value (Shah et al. 2019). Extrusion technique may be utilized to make a wide range of food items with lower processing costs, continuous production, high throughputs, and superior product quality while utilizing less energy (Prabha et al. 2021). Hence, extrusion cooking is considered an economical procedure that uses hightemperature and short-time technology (HTST) with low cost and reliability (Grasso 2020). In this extrusion process, the cooking temperature should be as high as 180-190°C; residence time is usually 20-40 s. These conditions during extrusion result in gelatinization of starch, denaturation of protein, inactivation of raw food enzymes, diminishing of microbial counts in the final product, and destruction of naturally occurring toxic substances like aflatoxin (Saalia & Phillips 2011). Extruded products are also gaining wide acceptability among all human ages. But the traditionally known corn extruded snacks are considered unhealthy due to their poor nutritional content. Since the extrusion enables the combining of different ingredients, it is also ideal for the creation of new functional foods (Shah et al. 2019). According to Who (2019), functional foods are any natural food popularly believed to promote good health by containing vital nutrients. Functional foods also include any food types enhanced by additives like cereals, bread, or beverages that are fortified with vitamins and herbs. Many lowcost but valuable medicinal herbs are easily available and are very useful due to their nutraceutical properties. Herbs not only enhance the taste and flavour of foods but their antimicrobial and antifungal properties also help to increase the shelf life (Salmerón-Manzano et al. 2020).
Laurus nobilis belongs to the Lauraceae family and is commonly known as a sweet bay but locally known as Alghar. Recent studies have shown that L. nobilis seed and leaf essential oils have narcotic, antibacterial, fungicidal, gastroprotective, antinociceptive, antidiabetic cytotoxic, anti-inflammatory and trypanocidal properties (Dadalioglu & Evrendilek 2004, Mohammed et al. 2021. For its flavouring and digestive characteristics, turmeric (Curcuma longa) is extensively consumed. Curcumin, a phenolic yellow pigment is the key active component of turmeric. It has been traditionally used for centuries as a remedy for various disorders including the common cold, wounds, ulcers and liver disease (Raduly et al. 2021). Also a number of studies have found that it can offer substantial benefits in a number of diseases, including heart disorders, diabetes, rheumatoid arthritis, Alzheimer's disease, inflammatory bowel illness, liver fibrosis and cirrhosis, HIV, pancreatitis, malaria and it is also reported to be a safe chemopreventive agent that are able to suppress cancer metastasis (Hewlings & Kalman 2017;Joe et al. 2004).
On the other hand, Zingiber officinale Roscoe, generally regarded as ginger, has been mostly utilized as both a seasoning and an herbal remedy across the world. It is considered one of the pungent taste herbs which contribute to the Zingiberaceae family. It has proven to display several therapeutic effects like antiplatelet antioxidant, anti-inflammatory, antimicrobial, antitumor and function (Karuppiah & Rajaram 2012;Mao et al. 2019).
This research, therefore, aimed to develop a new type of value-added extruded corn snacks through incorporating the above-mentioned herbs namely L. nobilis, C. longa and Z. officinale, which could provide consumers with various functional benefits. The nutritional, functional and physicochemical characteristics of these products were evaluated.

Raw materials
Yellow corn grits (Zea mays L.), was provided from "Egypt Food Company", Quisna, Minoufiya, Egypt factory delivered in the original package. Dried turmeric (Curcuma longa), ginger (Zingiber officinale Roscoe, Zingiberacae) and bay leaf or laurel (Laurus nobilis, Lauraceae) powder were purchased from the local market of Egypt.

Formulation of extruded snacks
Dehydrated powdered herbs of were added at 1, 3 and 5% level plus a mixture of all three herbs were prepared at percent 1:1:1 and were added by percent of 1, 3 and 5% to corn flour. A standardized amount of salt i.e. 2% was added to all treatments ( Table 1).

Development of extruded snacks
The extruded products were prepared at "Egypt Food Company" Quisna, Minoufiya, Egypt. A snack extruder (American extrusion) with a processing capacity of 150 kg per hour was used. The mixtures were blended for 10 min before extrusion. The mixtures then were mix-fed to the extruder. Extruder conditions used a moisture content of 13% and a temperature of 180°C with a rotation screw speed of 700 rpm were used for all blends. A 3 mm diameter die was applied. The feeding rate was approximately 3 kg/min. After that, the extrusion products were dried at (115 and 125°C) for 2 to 3 min by a pelt conveyor oven equipped with a diesel oil burner (Model 3000 Dryer American extrusion). After production, the corn snacks were cooled to room temperature (24°C) and were then packed in polyethylene bags.

Organoleptic evaluation of extruded snacks
A panel of semi-trained judges (15 panelists) carried out the organoleptic estimation of the formulated products. The treatments include 4 types of extruded products. Each contains three levels of herbs plus one control (without herbs). The nine-point hedonic scale for characteristics comprised appearance, colour, texture, aroma, taste and overall acceptance was used (Lawless & Heymann 2010). The exact composition of products was not revealed to the panelists to get their exact judgment of the samples. The mean scores for each product were then calculated. The proximate analysis The Proximate composition analysis (protein, ash, crude fiber, carbohydrates, and lipids) of both raw materials and the extruded products were estimated according to the AACC (2000).

Minerals and vitamins Estimation of minerals
Macro-minerals (calcium, magnesium, potassium, sodium, and phosphorus) and trace minerals (Zinc, iron, and copper) were estimated in both raw blends and extruded products. Briefly, the prepared Ash of the samples was boiled with 10 mL of 20% hydrochloric acid then diluted with deionized water after filtration as described by AOAC (2005). Then Minerals was measured using the atomic absorption spectrometry (AAS, Varian model: 220FS). Whereas, phosphorus was evaluated spectrophotometrically by the molybdate technique as described by Onwuka (2005).

Chromatographic determination of vitamins
Standard vitamins including water-soluble vitamin (pyridoxine-B6), and fat-soluble vitamin (vitamin A) were obtained from Sigma-Aldrich. All extruded samples were firstly grounded to a homogenous state using a food processor. Analysis of vitamin B6 was performed using HPLC by a commercial analytical laboratory (AgriQuality New Zealand). Briefly, enzymatic hydrolysis was employed for dephosphorylating the vitamins for the analysis of pyridoxine. The detection of pyridoxine was then assayed by reversed-phase, ion-pair HPLC (Bitsch & Möller 1989;Reitzer-Bergaentzle et al. 1993). Separation was accomplished isocratically with a mobile phase consisting of acetonitrile/0-05 M potassium dihydrogen phosphate (4:96, v/v) containing 0.5 × 10 3 M sodium heptane sulfonate. The mobile phase was then adjusted to pH 2.50 with phosphoric acid and filtered through cellulose acetate filter (0.45/zm). The separation was performed at ambient temperature at a flow rate of 1 mL/min. The fluorometric detector ran at an excitation wavelength of 290 nm and at an emission wavelength of 395 nm. For HPLC estimation of vitamin A, the saponification method provided by Dennison and Kirk (1977) was carried out. For HPLC analysis, an Eclipse × BD -C18 column (4.6 × 250 mm 5 μm) was used with a linear gradient of methanol: water (95:5) at a constant flow rate of 1 mL/min by using a binary pump with column temperature of 40°C. A multiple wavelength detector was employed for the detection of vitamins using a wavelength of 325 nm. The true retention of the vitamin content of the extruded product was calculated according to (Bergström 1998) based on the following equation.
Vita min content of extruded product Â g extruded ð Þ = Vita min content in raw blends Â g component ð Þ

Functional properties Extraction of active compounds
Phytochemical components were evaluated in both the extruded snacks and raw blended formula following the method of Horvat et al. (2020). Briefly, all snacks were milled and 1 g of each was mixed separately and homogenized with 3 mL of HCL in methanol (0.1%) for 2 min. The samples were then centrifuged (5 min at 4°C) at 7168 x g after 60 min of sonication (Sonorex Digitec, Bandelin, Germany, frequency 20 kHz, power 100 W). The retrieved supernatants were gathered and kept in the dark at − 20°C until analysis.

Total phenolic content (TPC)
The TPC in the previously collected supernatant was estimated following the technique of Singleton et al. (1999) with certain modifications. Briefly, 0.1 mL of the Folin-Ciocalteu phenol reagent (1:1) was mixed with 0.1 mL of sample or to the standard dilutions (100-500 μg/ mL) and 1.5 mL of distilled water. Upon homogenization for 5 min, it was vortexed with 0.3 mL of 20 g/100 mL Na 2 CO 3 solution. After that, it was kept away from light for 60 min at the ambient temperature. Then, the absorbance of samples was read at 740 nm spectrophotometrically (Cary 50-Varian Inc., Walnut Creek, CA, USA) against a blank which was acidified methanol. The experiment was done in triplicate. The findings were represented as mg gallic acid equivalents per g (mg (GAE)/g) of dry matter.

Total flavonoids content (TFC)
TFC was estimated following the colourimetric assay of Zhishen et al. (1999). 1 mL of extract solution and/or dilutions of a standard solution (10-100 μg/mL) was added to 4 mL of distilled water. 5 g/100 mL NaNO2 (0.3 mL) was applied to the latter mixture. After 5 min, 0.3 mL of 10 g/100 mL AlCl3 was added. After 6 min, 2 mL of 1 M NaOH was added. Finally dist. H 2 O was added to complete the overall volume to 10 mL and it was well blended. The absorbance of solutions was read against a freshly prepared blank at 510 nm. The TFC was expressed as mg quercetin equivalents per g of dry matter (mg QE/g).

Antioxidant activity of extruded products (AOXA)
The AOXA of both raw blended formula and its extruded snacks was conducted in accordance with Sanchez-Moreno et al. (1998). Thus, 3.9 mL of DPPH (1, 1-diphenyl-2-picryl hydrazyl, 0.025 g/L methanol) was mixed with 0.1 mL of each extracted sample.
After stirring and storing in the dark place for 15 min, the absorbance was recorded at 515 nm against a blank. The analysis was performed in triplicates. The scavenging activity was calculated by the following equation: Chromatographic determination of polyphenolic compounds All Commercial standards including 21 phenolic compounds were purchased from Sigma-Aldrich (USA). Both raw blends and snacks extrudates were powdered by using a laboratory homogenizer. Briefly, phenolic compounds were extracted from 0.5 g of the powdered samples using 70% methanol (Sigma) on an ultrasonic bath at 70°C for 3 h. Then, all samples were filtered. Each extract was evaporated to dryness. The residue was dissolved in methanol and used for HPLC analyses after filtration with a 0.45 μm syringe filter. HPLC Hewllet packared (series 1050) equipped with an auto-sampling injector, solvent degasser, and quarter HP pump (series 1050). For estimation of phenolic compounds, Gradient elution by using a mobile phase of solvent A (2% acetic acid) and solvent B (0.5% acetic acid: acetonitrile (1:1)) was used. Whereas for flavonoids, gradient elution was done by using a mobile phase of solvent A (2% acetic acid), and solvent B (methanol) was used. The gradients of the setup according to Marchev et al., (2011). The flow rate was 1 mL/min and the injection volume was 20 μL. UV absorbance was measured at 220-365 nm. Retention time and peak area (%) were used to identify and calculate the phenolic and flavonoids compound concentrations by comparing them with those of reference standards.

Physical properties of extruded snacks Expansion ratio and bulk density (BD)
The diameter of the snacks was taken as a mean of diameters of 15 pieces from each treatment (Alam et al. 2014). Then the expansion ratio (ER) and bulk density (BD) (g cm − 3 ) of the snacks were defined according to (Alvarez-Martinez et al. 1988) using the following equation: ER ¼ snack diameter mm ð Þ=die diameter mm ð Þ: Where m is the sample weight (g), D is the diameter of each snack (cm) and L is the length of the extrudate (cm).

Cross-section analysis through image j
The investigation of cell properties was performed according to Lotfi Shirazi et al. (2020) with some modifications. Cross-sections were made by cutting spheres of each extruded product in the middle with a Stanley knife A. Then it was captured by a colour digital camera (AQM-LX1) with a lens focal length of 35 mm and was located vertically at a fixed distance. The lens aperture of 5.6, ISO 320, and a shutter speed of 1/80 s with 3840 × 2160 pixels were used. The image analysis was firstly managed by the Photofiltre Studio software (version X). The center of images of the snack pieces was cropped at a size of 40 × 40 mm and then analysed by the ImageJ software (National Institutes of Health, USA version 1.53 c). The type of image was converted to grey-level (8 bits). After adjusting the contrast, the pixel scale was converted to mm by using a known length. In turn, a freehand selection tool was used to line the cells. Measurements were taken to elucidate the mean area of cells (mm2) and numbers of cells for each type of extruded product. Successively the area distribution figure was obtained.

Colour analysis
The extruded samples were grounded. Ground samples were sieved with a 60-mesh sieve to ensure a homogeneous sample. Five measurements were recorded for each sample with Chromameter (CR-400, Konica Minolta) (Singkhornart et al. 2014). The Lvalue in the colour system represented lightness with zero for darkness and 100 for lightness; a value represented the extent of green colour in the range from − 100 to zero and red in the range from zero to 100; b-value quantifies blue in the range from − 100 to zero and yellow in the range from zero to 100.

Instrumental texture analysis
A Texture Analyzer (TA.XT Plus, UK) with a 5 kg cell load and a cylindrical probe of 35 mm in diameter was used for estimating the texture features of the corn extruded sample. Parameters used in this study were: pretest speed, 1.00 mm/s; test speed, 1 mm/s; post-test speed, 2 mm/s; data processing rate, 200 points/s. Hardness was derived as the maximum peak force, measured in Newton (N). Whereas, adhesiveness is the negative force area A3 estimated for the first bite (Mazumder et al. 2007). For calculating other parameters, the following equations were used.
Springiness is defined as how much a product physically springs back after deformed during the first pressure and could wait between the two strokes. This test was run in triplicate for each extruded Microbial analysis 0.5 g was taken from each sample for the microbiological test. Total bacterial count Agar (Biolife, USA) was utilized for counting of total count bacteria (TBC), while potato dextrose agar (Biolife, USA) was exploited for yeast and mould counts (YM). The total coliform count was done on Violet Red Bile Agar (VRBA, Biolife, USA) and incubated at 35°C for 48 h. All counts were calculated as CFU/g. According to Salfinger and Tortorello, (2017), in all extruded products during storage for 9 months in a closed container. All findings were expressed as an average of three replicates.

Statistical analyses
All experiments were repeated three times and the related analysis results are expressed as average. The associated results were subjected to the analysis of variance (ANOVA) by the Statistical Analysis System SAS (2017). Duncan's multiple range analysis was used to distinguish the relevance of the discrepancy at p < 0.05. The variation in the significance is indicated by different series of superscripts (e.g., a, ab, b,. . z). The correlation between different parameters was done with Excel.

Sensory evaluation of extruded snacks
Different treatments of extruded products containing varied ratios ranging from 1 to 5% of each herb and the mixture of the three herbs (1:1:1) plus a control extruded product were organoleptic evaluated ( Table 2). As can be noticed, the laurel-corn snacks gave the best odour in the lower concentrations up to 3% with desired long-lasting mouthfeel effect. While turmeric-corn snacks gave the best colour as well as flavour. The ginger treatment exhibited some spicy taste which increased with increasing the concentration, while the treatment included mixtures of herbs comprising all the flavours of its component, and it was sensory accepted at law concentration (3%). The overall evaluation indicated that the best score is for the concentration of 3% of each herb and also of the treatment containing herb mixtures ( Fig. 1).

Proximate analysis
The proximate composition of both raw components and the extruded snacks are presented in Table 3. As can be shown, there was little difference in protein content between treatments (p > 0.05), and this was somewhat improved relative to control after the inclusion of various herbs being the highest in T4. The moisture content of products is greatly affected by the extrusion process and its range was approximately between 5 and 6%. The maximum moisture level of extruded items was for T3. The fat level was marginally modified and the maximum content was in T4. While the ash content was increased significantly in the extruded products containing herbs than in the control sample being the highest in T2 and T4. As well crude fiber content was slightly raised for all treatments and the highest content was detected in the mixture treatment (T4). Meanwhile, the highest carbohydrate content was recorded by the control sample.

Minerals and vitamins
When raw blends were compared to extruded products (Table 4), it was revealed that all treatments improved in mineral content with the exception of Zn in control, K in T1, Mg in T2, Ca in control, and P in all treatments with the exception of T2, which exhibited a minor increase. However, when extruded products were compared to the control, there was a significant increase in mineral content (Fig. 2). Fe, K, Ca, and Zn had the biggest percentage rise in herbal corn snacks, ranging from 48 to 505.7%, 4.1 to 497%, 97.05 to 176.4%, and 108.2 to 163.3%, respectively. It can be shown that the contents of K, Ca, and Fe in T4 get the highest percentage of increase. Table 5 indicates the vitamin content of extruded items, whereas Fig. 3 illustrates vitamin retention following the extrusion process. The vitamin A content of the herbal extruded items ranged from 283 IU to 445 IU/ 100 g. The turmeric-extruded product T2 has the  greatest vitamin A content with 12.29% vitamin retention percent, followed by the mixture-extruded product (335 IU) but with a retention percent of 21%. The levels of vitamin B6 (Pyridoxine) in the herbal extruded preparations varied from 0.01 to 0.08 mg/100 g. T2 extruded product had the greatest vitamin B6 content, followed by T4 extrudate, with vitamin retention of 86.21 and 75%, respectively.

Functional properties
Certain functional attributes were assessed in the extruded products including overall phenolic, flavonoid contents and scavenging activity in comparison to raw blends prior to extrusion ( Table 6). The extrusion process primarily resulted in a reduction in the active compounds. The loss percent is ranged from 84.5 to 14% for TPC being the highest in the control and the lowest in T2 treatment. Even though for TFC, there was an increasing percent of 32 and 7% for control and T1, respectively, the percent loss was also recorded for other treatments which were varied from 50 to 64%. For AOXA, the increasing percent for control was 20%, while the loss percent in treatments was ranged from 15 to 40% being the highest in T4 and the lowest in T1. For extruded products, the maximum TPC was recorded for T2. While the highest levels of TFC and AOXA were noted for T1. The change in functional properties was also expressed as an increasing percent between the extruded items as compared to control-extruded snacks (Fig. 4). It was observed that all the parameters analysed had been improved. For TPC, the increased percent were ranging from 742.62% (T2) to 451.79% (T1), from 188.55 (T1) to 17.63% (T3) for TFC, and from 313.29 (T1) to 98.74% (T3) for AOXA.
The changes in individual phenolics before and after extrusion are presented in Table 7. HPLC detected only 10 phenolic acids in corn snacks compared to 17, 18 and The largest retention percent was found for cinnamic acid (714%) and was detected in T2.

Physical characteristics of extruded snacks blends
The colour analysis presented in Table 8 showed values ranged from 74.5to 91.93 for L*, and from 5.5 to − 3.79 for a*, and from 27.66 to 43.02 for the b* in all corn snacks. The highest L* value (91.93) was observed in control-corn snacks followed by T3 (80.99). The lowest L* value (74.5) was noted in T1. Regarding the redness degree, the highest a* value (5.5) was obtained in control-corn snacks followed by T3 (2.5), while other treatments took the negative value indicating the greenish degree and the maximum negative a* value were for T1 (− 3.79). For b* value, the highest yellowish degree was apparent in T2, followed by T4, whereas the lowest b* value was noted with T3. Other treatments took a median yellowish value indicated by the low hue value (h). Higher values of ΔE were obtained with T2 followed by T1. Whereas the colourfulness intensity, chroma (C*) was the uppermost in T4 followed by T2.
As demonstrated in Table 8, supplementation of corn flour with dried herbs showed a significant impact on expansion ratio as well as piece density of the extruded corn snacks. T2 had the highest value for expansion ratio (4.02), while T3 had the lowest value (2.83). The extruded-corn snacks had a bulk density's value ranged from 0.35 to 0.12 g/cm 3 . The maximum bulk density's value was for T2, whereas the lowest was for controlcorn snacks and T2. The largest open-cells emerged in Raw blends: blends prepared before extrusion process. Control: plain extruded product. T1, T2, T3 and T4: extruded corn product fortified with 3% (w/w) of laurel, turmeric, ginger and mixture formula contains 3% of the three herbs' mixture mentioned (1:1:1), respectively Fig. 3 The vitamin retention percent of extruded products as compared to raw blends. Control: plain extruded product. T1, T2, T3 and T4: extruded corn products fortified with 3% (w/w) of laurel, turmeric, ginger and mixture formula contains 3% of the three herbs' mixture mentioned (1:1:1), respectively T2 with a reduced number as indicated by the crosssection view; nevertheless, the ginger snack (T3) showed many little air cells (Table 8, Fig. 5).
The rheology properties of extruded corn snacks are represented in Table 9. It was indicated that the texture analysis for snacks exhibited a hardness values ranged from 24.17 (T3) to 18.09 N (T1). Maximum adhesiveness (0.3) was recorded for the control sample and T4, while the lowest value was observed with T1 and T2. It was also noticed that springiness, gumminess and chewiness gave the highest value in T1 and the lowest value in T3. Regarding resilience, the maximum value was noted in the control-corn snacks, whereas the minimum value was observed in T4.

Microbial analysis
The microbial load of extruded-corn snacks during storing for 9 months at room temperature was very low to count. The findings indicate that both TBC and Y&M were missing for 6 months. After 9 months of storage, fewer TBCs emerged in the control group alone (2.3 log CFU/g). Also, fewer yeasts and mold colonies started to appear in extruded samples but it was relatively higher in the control sample (2.95 log CFU/g) than in the other herbal corn snacks (ranging from 1 to 1.3 log CFU/g). As well the coliform group was not detectable in all extruded products during storage periods. In T4 treatment, there was a complete absence of TBC and Y&M during the period of storage.

Discussion
Extruded food products are accepted by the public of all ages. But these products have low functional value (Prabha et al. 2021). The present study consolidated extruded snacks of corn with various herbs including bay AoxA: total antioxidant activity; TPC: total phenolic content; TFC: total flavonoid content (mg/100 g). T1, T2, T3 and T4: extruded corn product fortified with 3% (w/ w) of laurel, turmeric, ginger and mixture formula contains 3% of the three herbs' mixture mentioned (1:1:1), respectively Fig. 4 The increasing percent of functional properties in treatments as compared to control. Control: plain extruded product. T1, T2, T3 and T4: extruded corn products fortified with 3% (w/w) of Laurel, turmeric, ginger and mixture formula contains 3% of mix of the three herbs used (1:1:1), respectively  leaves, ginger, turmeric, and a mixture of them. These herbs were added by different ratios and the concentration of 3% of each treatment was selected according to the sensory properties defined by the judges. The inclusion of laurel leaves gave the highest score in flavour in snacks, which is linked to the richness of laurels with volatile oil. The principal volatile oils in bay leaves are 1,8-Cineole (31.9%), sabinene (12.2%), and linalool (10.2%) (Caputo et al. 2017). The highest colour score was the turmeric-extruded snacks. Turmeric is considered a major source of polyphenol curcumin which gives it a spectacular yellow hue (Hewlings & Kalman 2017). Whereas, ginger-extruded products have a pungency flavour, which develops primarily from its gingerol content, that when heated becomes dehydrated creating pungency compounds known as shogaols (Mao et al. 2019).
On the other hand, the combined herbs-corn snacks (T4) have collected all the properties of the colour and taste of its herbal ingredients. It comes with a yellowgreen colour, rich taste with little pungency which can be preferred by children and young adults alike. The nutritional value is considered the first concern in any commercial product. Moisture is a critical parameter in snack evaluation as it has contributed to the crispiness of the products (Yadav et al. 2018). The moisture levels of this study are close to those recorded by Goda et al. (2019). It has been found that ginger corn snacks represented the highest moisture content that was substantially different from other extruded types, which may be attributed to the water absorption potential of ginger as stated by Awad (2018). It was observed that all herbal extruded corn snacks had comparatively higher ash and fiber content with a lower content of carbohydrate compared to control. And these findings are consistent with Kaur et al. (2018) as they noticed the same results after using herbs like mint and basil. This relates to the alternation of starch content with other herbal ingredients such as fibers and minerals, which can be a promoting for weight loss.
Minerals have a vital role to play in human nutrition. The three spices used in developed extruded products; ginger, turmeric, and bay leaves have been established as important sources of various mineral elements such as potassium, magnesium, phosphorous, calcium, iron, and trace amounts of zinc (Guenane et al. 2016;Nobile-Correa et al. 2020;Tanweer et al. 2014). Herbal corn snacks developed in the current study have shown that almost all types of minerals have risen after extrusion, with a marked increase in Fe, Ca, K, and Zn content. This percent of the increase in mineral content was higher than what reported by Kaur et al. (2018) which was in the range of 19-29, 38-77 and 52-63% for total iron, calcium, and zinc content in the extruded product, respectively. Minerals are stable chemical elements. Although there is no clear explanation until now for the mineral behavior during extrusion, the reported increase in minerals after extrusions could be explained on the basis of degradation of phytate. Phytate is hydrolyzed and its phosphate molecule is released and inactivated, and this triggers the release of these elements (Alonso et al. 2001). Mineral elements are considered to be essential at all stages of human development. Calcium is required not just for bone formation throughout infancy and adolescence, but also for osteoporosis in postmenopausal women (Eastell et al. 2019;Levine 2012) the current manufactured herbal exrudated could provide a percent ranging from 29 to 35% of the daily required value (DV) for calcium recommended by FDA (2016). To the same extent, premenopausal women and small children are the most vulnerable to iron deficiency. Iron deficiency can impair behavior and learning ability (Allen, 2000). The herbal corn snacks contain 40.5-290 mg/g of iron, which surpasses the recommended DV of 18 mg (FDA 2016). Zinc has lately been advised to help combat the common cold, boost immunity, reduce diarrhea, and promote normal growth during pregnancy, infancy, and adolescence (Maret & Sandstead 2006). The DV for zinc is 11 mg (FDA 2016), thus the herbal corn snacks might supply a DV percent of around 90% per gram of snacks. Potassium is thought to have an important function in the control of skeletal muscle contraction, hypertension, reducing hypercalciuria and nerve impulse propagation (Stone et al. 2016). The current manufactured herbal corn extruded product could L*: lightness; a*: red-green balance; b*: yellow-blue balance; ΔE: total colour chance index, h: Hue angle and ΔE: Total colour difference, a-c: indicated with similar letters in columns do not differ significantly at p = 0.05. T1, T2, T3 and T4: extruded corn product fortified with 3% (w/w) of laurel, turmeric, ginger and mixture formula contains 3% of the three herbs' mixture mentioned (1:1:1), respectively supply a DV % of potassium ranging from 11.7 to 68% (FDA 2016). According to the FDA, foods that provide 20% or more of the DV are considered rich sources of a nutrient, although items that provide lower percentages of the DV also contribute to a healthy diet. Vitamins are also known to be an integral part of the diet (Godswill et al. 2020). Vitamin B6 is among watersoluble vitamins, is considered as an enzymatic co-factor that is integrated into numerous biochemical reactions such as biosynthetic pathway of amino acids, and metabolic process of sugar, and fatty acid (Ball 2006). Vitamin B6 has been found to be also a potent antioxidant (Sorolla et al. 2010). Scientific investigations have shown that DNA damage caused by micronutrient deficiencies, such as vitamin B6, may lead to stunted growth, anemia, poor immune function, and cancer (Liu et al. 2008;Qian et al. 2017). B6 is regarded as the most heat-sensitive (Killeit 1994). Although some researchers reported stability of B6 during extrusion, others reported a loss (Yang et al. 2020). In the current results, the vitamin retention percent for B6 was ranged from 21 to 78%. It seems that this effect depends not only on the production process but also on the food mixtures used. These findings are consistent with a variety of authors. For instance, Athar et al. (2006) recorded a vitamin B6 retention percent of 18 and 35% for the corn-peas mixture and the extruded oat flour, respectively.
Vitamin A is essential for proper immune functions and the deficiency of this vitamin is the leading cause of blindness in many developing countries. Vitamin A and related carotenoids are not stable in the presence of oxygen and heat and are thus particularly vulnerable during extrusion. Because vitamin A contains four double bonds and one hydroxyl group, which are unstable at high temperatures (Athar et al. 2006;Kostadinović et al. 2014). Vitamin A content in turmeric rhizome powder was reported to be 3.44 mg/g (Imoru et al. 2018). The prepared herbal extrudates could cover a DV% ranged from 14 to 9.4% of the daily value of vitamin A recommended by FDA (2016). Although the turmeric-raw blends showed the highest content in vitamin A, they displayed the lowest percent retention after extrusion. The retention of vitamins in cereal during the extrusion process is not apparently linked to the original levels of the vitamins (Athar et al. 2006). Yang et al. (2020) rated vitamin stability during extrusion and stated that vitamin B6 was relatively stable, while vitamin A was very sensitive.
Because of their known antioxidant characteristics, the phytochemicals in cereals attract considerable scrutiny owing to their worthy impact on human health (Horvat et al. 2020). In L. nobilis extracts, the total phenols and flavonoids were previously stated to be 25.70 ± 0.86 mg GAE/g and 12.11 ± 0.43 mg CE/g, respectively (Guenane et al. 2016). In the case of ginger, it was informed to be 52.8 μg GAE/mg and 3.9 μg QE/mg, respectively (Tohma et al. 2017). Whereas it ranged from 3.8 to 221 mg GAE/ g and 0.6 to 549.2 mg QE/g for turmeric extracts, respectively. It is already established that the antioxidant properties of phenolic compounds depends on the ability to scavenge free radicals, to give hydrogen atoms, electrons, or chelate metal cations (Afanas'ev et al. 1989). In the extrusion process, heat introduces two different effects on phenolic molecules, the first being the increase in the phenolic levels caused by the liberation of these substances out of the cell membrane/wall under the influence of heat (Dewanto et al. 2002). While the other effect is the modification or the destruction of the chemical structure of these compounds that will eventually lead to a reduction in their quantity and activity, due to the exposure to high heat and time of exposure during the extrusion process (Vega-Gálvez et al. 2012). That reduction is not often correlated with the same proportion of the decline in the antioxidant capacity as the Millard reaction could accumulate other antioxidant molecules that would decrease such loss (Vega-Gálvez et al. 2012). Decreased functional parameters have also been documented in other extruded items that differ by food type (Limsangouan et al. 2010) and a reduction of 80% of phenolic content has been reported (Repo-Carrasco-Valencia et al. 2009). Also, the results showed a strong positive correlation between antioxidant activity and total flavonoids (r = 0.94), but its relationship with polyphenolic content was moderately high (r = 0.65). A lack of correlation (r = 0.20) between TPC and DPPH was indicated to occur in corn samples (Horvat et al. 2020). The utilization of herbs in this study may be the explanation for the moderate relationship appearance.
In the current study, the extrusion cooking did not introduce an alteration in phenolic profiles but only change its concentration. The predominance of ferulic acid and catechin in all extrudates is related to the richness of yellow corn of these compounds. Herein, introducing herbs to corn increases the phenolic compounds in extrudates. This is obviously noticed by the detection of gingerol and curcumin in ginger and turmeric snacks. Besides including both of these compounds in herbalmixture snacks. Gingerol is known for its antioxidant, anti-tumor and anti-inflammatory properties. Also it can reverse or prevent chronic diseases (Aryaeian & Tavakkoli 2015). Many studies have publicised the health benefits of curcumin, demethoxycurcumin and bisdemethoxycurcumin, which are main active compound in turmeric such as anti-bacterial, anti-viral, anti-oxidant, anti-inflammatory and anticancer activities (Jayathilake et al. 2021;Rathore et al. 2020).
The first physical characteristic that draws customers is color (Turner 1995). The incorporation of diverse herbs expressly led to a distinct coloration in the achieved study. The presence of carotenoid in corn flour gives the control-corn snacks a light yellow color (Kannadhason & Muthukumarappan 2010, Shah et al. 2017. The most noticeable color shift was that of turmeric, which displayed a golden color that naturally resulted from its curcumin concentration (Nobile-Correa et al. 2020). In addition, bay laurel has a more greenish color due to its chlorophyll concentration (Ayanoğlu et al. 2018). Lightness L reduced somewhat after herbal addition, and the similar tendency was reported in fruit-corn snakes by Wójtowicz et al. (2020). The total color difference, on the other hand, defines the amount of color difference between treated and control samples (Mokrzycki & Tatol 2012). The greatest difference was observed with turmeric snacks. Furthermore, chroma (C*) is regarded as a quantitative feature of colorfulness, and according to the results, turmeric and a mixture of herbs-snacks exerted the highest color intensity. Since the hue angle (h*) represented the qualitative quality of color, a lower hue angle indicates a more yellow character. The hue angle range achieved is similar to that found with spirulina-extruded snacks, which are reported to have a green hue ranging from 85 to 92 (Lucas et al. 2017). Different processes, such as the Millard reaction, caramelization, hydrolysis, and pigment degradation, may also have an effect on the degree of color (Kannadhason & Muthukumarappan 2010).
The expansion ratio is an important parameter in the extruded product. The expansion ratio depends on different factors like initial moisture, type of fibers, and protein contents (Minweyelet et al. 2021). Herein the ginger snacks showed less expansion ratio, which may be related to the higher moisture content. The plasticizing effect of the water makes a reduction in starch viscosity that restricts the bubble growth so producing many small air cells with low surface area (Ding et al. 2005) and that like what observed in cross section of ginger snacks. On the same trend, the presence of high fiber and protein content will results in a further harder, less expanded and denser extruded product (Chou & Hsu, 2020) and that comparable with what observed in laurel and ginger snacks. Oppositely, the addition of turmeric in corn snacks led to a more expanded product than other extruded products and with a density value closer to control. Turmeric powder has starch in its composition, distinguished by its smooth surface (Tejavathi et al. 2020). This turmeric-starch has described as having a high range of gelatinization and viscosity (Santana et al. 2017), which would lead to further swelling of the dough allowing for an extra expanded softer surface which is also noted in cross section giving the largest cell area. The obtained range of bulk density is closely similar to that found by Ding et al. (2005) which was between 0.10 and 0.43 g/cm 3 for extruded rice-based snacks. As well shah et al. (Shah et al. 2017) reported values ranged from 2.64 to 5.72 and from 0.34-0.44 g/cm3 for expansion ratio and the piece density, respectively.
Texture parameters are known to be major challenge faced by extruded puff products. Hardness refers to the peak force. A product with a lower density, lesser hardness and a highly porous structure is preferred for crispy extrudates (Saeleaw et al. 2012). There was no big difference in the hardness values of the current products. Nevertheless, the ginger snacks score a superior hardness value compared to other extrudates. That may be related to the higher water content in ginger snacks which will also result in a decrease in the expanding level (Petrova et al. 2010). Adhesiveness is the tendency of the food to stick to the teeth as it is chewed. This property is known for the buffed products (Liu et al. 2000). Researchers have reported that the stickiness of the product is similarly increased with high moisture content (Shruthi et al. 2019) that is clearly noticed with ginger snacks in the obtained findings. In crispy products, the ability to spring back is less and that is evident in the current results in the herbal-mixture snacks. Likewise, resilience explains how much a product can struggle for recovery but if the product is crispy it will get break and show less resilience (Leonard et al. 2020). Approximately all treatments showed a lower value of resilience in comparison to control. Springiness in the current results is positively linked to gumminess (r = 0.99) and chewiness (r = 0.96). Also, gumminess is strongly correlated with chewiness (r = 0.95). These parameters are related with each other as they describe how the product behaves during bites. However, hardness gave a clear downhill relationship with gumminess, springiness and chewiness(r = − 0.98, − 0.96 and-0.91 respectively) which have similarities with the findings of Liu et al. (Liu et al. 2000) and Shah et al. (Shah et al. 2017).
Microbiological load indicated the quality of handling and storage circumstances; all extruded products were determined to be within permissible limits during storage at room temperature for 6 months for the control sample and 9 months for the herbal extruded products (ICMSF 2011). Because the high temperature utilized during extrusion is high enough to eradicate microbiological dangers, which may be comparable to the sterilizing procedure (Syed et al. 2019;Wani et al. 2020). The lack of yeast and molds during storage is familiar with the finding of (Morsy et al. 2014). Furthermore, the coliform group was missing from all analysed samples, which was similar with the findings of other authors (Syed et al. 2019, Wani et al. 2020. However, the current complete lack of total bacterial counted over 9 months for herbal extruded snacks outperforms the findings of other authors who reported lower total counts of bacteria, yeast, and molds (Raja et al. 2014;Vijayarani et al. 2012). And that could be associated with the antimicrobial activity exhibited by the currently used herbs. Since, polyphenols of bay leaves, ginger, and turmeric were confirmed to have potent antimicrobial activity (Joe et al. 2004;Dadalioglu & Evrendilek 2004, Beristain-Bauza et al. 2019

Conclusions
The production of healthier foods is presently the main focus of the industrial process. The most obvious conclusion from this study is that two extruded products which is turmeric and herbal mixture snacks with the highest mineral content, including potassium, calcium, zinc, and iron, as well as the highest levels of vitamin B6 and A, may be regarded a rich nutritious diet. It also had a higher phytochemical content and better rheological qualities than the standard product. Furthermore, the extended shelf life of these herbal corn snacks assures that the product is microbiologically safe. Thus, including the aforementioned dry herbs into extruded maize snacks could make a significant contribution to the field of functional food.