Breeding Rice lines for physio-functional food through indica ‘Zhaxima’ × japonica ‘Nanjing 46’ haploid technique

Tian, Q. Q.; Li, X.; Lu, C. M.; Fang, X. W.

doi:10.1186/s43014-019-0010-7

Food Production, Processing and Nutrition

Table 2 Effects of different hormone components and treatment times on callus induction

From: Breeding Rice lines for physio-functional food through indica ‘Zhaxima’ × japonica ‘Nanjing 46’ haploid technique

Days after cold pretreatment(d)	Sampling area of spikes	Callusing induction rate of plated anthers (%) anthers (%)
Days after cold pretreatment(d)	Sampling area of spikes	IM-1	IM-2	IM-3	IM-4	IM-5	IM-6	IM-7
0	Top	6.21 ± 0.21b	1.91 ± 0.05e	7.82 ± 0.21a	2.53 ± 0.08d	2.61 ± 0.07d	1.31 ± 0.03f	3.61 ± 0.10c
	miiddle	6.51 ± 0.21b	2.05 ± 0.08e	8.03 ± 0.24a	2.84 ± 0.08d	2.81 ± 0.07d	1.41 ± 0.04f	3.81 ± 0.11c
	Bassal	8.03 ± 0.29a	2.92 ± 0.11d	8.12 ± 0.22a	3.13 ± 0.08c	3.01 ± 0.07d	1.51 ± 0.03e	4.11 ± 0.12b
1	Top	8.21 ± 0.29b	6.05 ± 0.19c	12.03 ± 0.33a	4.03 ± 0.12e	4.41 ± 0.13d	4.02 ± 0.12e	4.52 ± 0.13d
	miiddle	8.52 ± 0.31b	6.71 ± 0.23c	13.14 ± 0.33a	4.52 ± 0.17e	4.82 ± 0.11d	4.12 ± 0.12f	4.82 ± 0.11d
	Bassal	9.32 ± 0.32b	7.65 ± 0.20c	14.03 ± 0.32a	4.91 ± 0.14d	5.02 ± 0.11d	4.22 ± 0.11e	5.04 ± 0.15d
2	Top	9.33 ± 0.31c	9.11 ± 0.26c	12.51 ± 0.25b	7.05 ± 0.19e	15.05 ± 0.35a	8.03 ± 0.24d	8.06 ± 0.14d
	miiddle	9.51 ± 0.33e	11.02 ± 0.34c	19.05 ± 0.34a	7.52 ± 0.22 g	15.51 ± 0.36b	10.03 ± 0.17d	9.03 ± 0.27f
	Bassal	10.05 ± 0.35e	12.06 ± 0.38d	13.73 ± 0.31b	7.63 ± 0.22f	16.04 ± 0.38a	12.03 ± 0.26d	12.81 ± 0.51c
3	Top	10.54 ± 0.39f	14.02 ± 0.46d	23.45 ± 0.43b	10.03 ± 0.30 g	28.04 ± 0.74a	18.04 ± 0.44c	12.03 ± 0.36e
	miiddle	11.04 ± 0.41 g	15.04 ± 0.40d	32.14 ± 0.26a	12.10 ± 0.26f	25.04 ± 0.77b	20.03 ± 0.41c	13.02 ± 0.39e
	Bassal	11.51 ± 0.36d	16.41 ± 0.45b	22.83 ± 0.41a	14.03 ± 0.32c	22.14 ± 0.86a	22.04 ± 0.46a	14.03 ± 0.42c
4	Top	13.20 ± 0.41d	17.03 ± 0.48b	12.11 ± 0.33e	13.06 ± 0.39d	18.02 ± 0.54a	12.03 ± 0.26e	14.04 ± 0.42c
	miiddle	14.03 ± 0.45e	17.51 ± 0.30c	18.52 ± 0.37b	14.06 ± 0.32e	19.03 ± 0.47a	14.02 ± 0.42e	15.04 ± 0.45d
	Bassal	16.04 ± 0.54c	18.71 ± 0.44b	12.93 ± 0.28e	15.03 ± 0.35d	20.03 ± 0.51a	15.03 ± 0.45d	16.03 ± 0.48c
5	Top	18.12 ± 0.54a	18.02 ± 0.52a	11.92 ± 0.35b	8.22 ± 0.24c	12.03 ± 0.36b	4.21 ± 0.11d	2.62 ± 0.07e
	miiddle	18.54 ± 0.54a	18.52 ± 0.44a	12.51 ± 0.31b	8.41 ± 0.15d	11.02 ± 0.33c	4.43 ± 0.13e	2.71 ± 0.08f
	Bassal	19.91 ± 0.69a	18.93 ± 0.45b	12.72 ± 0.29c	8.61 ± 0.25e	10.52 ± 0.21d	4.81 ± 0.14f	3.02 ± 0.09 g
6	Top	15.33 ± 0.46b	16.03 ± 0.44a	10.07 ± 0.30c	6.01 ± 0.18d	6.04 ± 0.24d	3.03 ± 0.08e	2.41 ± 0.06f
	miiddle	16.51 ± 0.56	16.51 ± 0.46	16.04 ± 0.26	6.54 ± 0.19	6.21 ± 0.17	3.21 ± 0.08	2.51 ± 0.07
	Bassal	18.03 ± 0.62a	17.03 ± 0.51b	14.09 ± 0.32c	7.01 ± 0.20c	6.71 ± 0.13e	3.41 ± 0.10f	2.81 ± 0.08 g
7	Top	5.51 ± 0.19b	10.03 ± 0.35a	4.52 ± 0.11e	5.02 ± 0.15d	5.81 ± 0.17c	2.02 ± 0.05f	2.02 ± 0.06f
	miiddle	6.03 ± 0.22b	12.03 ± 0.38a	4.61 ± 0.13d	5.51 ± 0.16c	6.05 ± 0.17b	2.23 ± 0.06e	2.21 ± 0.06e
	Bassal	6.52 ± 0.21b	13.11 ± 0.42a	4.81 ± 0.14d	6.02 ± 0.18c	6.23 ± 0.17c	2.41 ± 0.07e	2.42 ± 0.07e
8	Top	5.02 ± 0.20b	8.03 ± 0.22a	4.04 ± 0.12c	3.02 ± 0.09d	5.06 ± 0.15b	0.81 ± 0.01f	1.81 ± 0.04e
	miiddle	5.11 ± 0.19c	8.51 ± 0.24a	4.22 ± 0.12d	3.24 ± 0.08e	5.51 ± 0.11b	1.02 ± 0.02 g	2.03 ± 0.06f
	Bassal	5.52 ± 0.19c	9.04 ± 0.26a	4.42 ± 0.13d	3.43 ± 0.09e	6.05 ± 0.11b	1.21 ± 0.04 g	2.31 ± 0.06f
9	Top	4.41 ± 0.17b	6.51 ± 0.16a	3.81 ± 0.09c	1.42 ± 0.04e	4.51 ± 0.13b	0	1.71 ± 0.04d
	miiddle	4.51 ± 0.17c	7.01 ± 0.18a	4.03 ± 0.11d	1.51 ± 0.04f	5.04 ± 0.14b	0	1.91 ± 0.04e
	Bassal	4.91 ± 0.18c	7.52 ± 0.22a	4.21 ± 0.12d	1.61 ± 0.03f	5.22 ± 0.14b	0	2.12 ± 0.04e
10	Top	0	0	2.01 ± 0.08b	1.21 ± 0.03d	2.81 ± 0.08a	0	1.32 ± 0.03c
	miiddle	0	0	2.31 ± 0.02b	1.32 ± 0.02c	3.02 ± 0.09a	0	1.21 ± 0.03d
	Bassal	0	0	2.24 ± 0.03b	1.42 ± 0.04c	3.11 ± 0.09a	0	1.41 ± 0.03c

Note: The media consisted of M8 basal medium (Mei et al. 1988) supplemented with 60 g•L⁻¹ sucrose and 8 g•L⁻¹ agar and different hormone complements as follows: IM-1: 1 mg•L⁻¹ 2,4-D and 3 mg•L⁻¹ NAA; IM-2: 1.5 mg•L⁻¹ 2,4-D and 2 mg•L⁻¹ NAA; IM-3: 1.5 mg•L^− 1 2,4-D, 2 mg•L^− 1 NAA, and 0.3 mg•L^− 1 KT; IM-4: 2 mg•L^− 1 2,4-D and 1 mg•L^− 1 NAA; IM-5: 2 mg•L^− 1 2,4-D, 1 mg•L^− 1 NAA, and 0.3 mg•L^− 1 KT; IM-6: 3 mg•L^− 1 2,4-D and 1 mg•L^− 1 NAA, IM-7: 3 mg•L^− 1 2,4-D and 0.5 mg•L^− 1 NAA. 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthylacetic acid; KT, kinetin; ABA, abscisic acid, IM, induction medium. Different letters above the bars indicate significant differences (P < 0.05).

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