Fig. 5

Histopathological and immunohistochemical analysis of lung samples. a and b represent histopathological findings of lungs collected from Bama miniature pigs in the M. hyopneumoniae strain JS-infected group (BI) and control group (BC) at 200 × magnification, respectively. c and d are histopathological findings of lungs collected from the conventional pigs in M. hyopneumoniae strain JS-infected group (CI) and control group (CC) at 200 × magnification, respectively. All pictures were captured with the microscope of Nikon (TI-S-EJOY). e and f represent immunohistochemical observation of lungs against M. hyopneumoniae antigen P97 collected from Bama miniature pigs in the M. hyopneumoniae strain JS-infected group (BI) and control group (BC), respectively. Brown staining appeared around inner edge of bronchial lumen in the M. hyopneumoniae strain JS-infected group of Bama mininature pigs against P97R1 and was marked by short black arrows (e). g and (h) are immunohistochemical observation of lung against M. hyopneumoniae antigen P97 collected from conventional pigs in the M. hyopneumoniae strain JS-infected group (CI) and control group (CC), respectively. Similarly, brown staining appeared in group CI against P97R1 and is marked by long black arrows (G), and the enlarged image of the M. hyopneumoniae antigen P97-stained area pointed by the arrow (captured at 200 × magnification) is shown in the upper right corner (E and G). The black bar represents the scale: 25 μm