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Table 4 Antioxidant properties, antioxidant activity and regression analysis of hypogeal germinated brown rice

From: Symbiotic and adverse interplay of hypogeal germination periods on brown rice (Oryza sativa): nutrient and non-nutrient characteristics

(a) Expt. No.

Run Order

Hypogeal Germination Period

DPPH

MCA

FRAP

TPC

TFC

  

(h)

(%)

(%)

(mMolFe2+)

(mgGAE/g)

(MgRUTIN/g)

1

10

0(−1)

35.30 ± 0.84

52.99 ± 0.29

0.054 ± 0.002

0.770 ± 0.005

50.63 ± 0.92

2

2

0(−1)

36.00 ± 0.21

52.99 ± 0.71

0.054 ± 0.001

0.770 ± 0.003

50.63 ± 0.48

3

3

18(−0.5)

37.01 ± 0.14

46.06 ± 0.32

0.096 ± 0.001

0.657 ± 0.004

45.08 ± 0.32

4

9

54(0.5)

40.85 ± 0.32

47.61 ± 0.47

0.121 ± 0.003

0.671 ± 0.006

45.10 ± 0.14

5

7

72(1)

43.60 ± 0.12

56.10 ± 0.46

0.106 ± 0.002

0.798 ± 0.002

50.17 ± 0.74

6

4

72(1)

43.60 ± 0.11

56.10 ± 0.38

0.106 ± 0.004

0.798 ± 0.009

50.17 ± 0.61

7

5

36(0)

38.80 ± 0.31

44.28 ± 0.14

0.118 ± 0.001

0.624 ± 0.004

43.47 ± 0.18

8

6

36(0)

38.00 ± 0.42

44.28 ± 0.73

0.118 ± 0.003

0.624 ± 0.006

43.47 ± 0.43

9

8

36(0)

38.00 ± 0.15

44.28 ± 0.28

0.119 ± 0.002

0.624 ± 0.003

43.47 ± 0.54

10

1

36(0)

38.00 ± 0.21

44.30 ± 0.51

0.118 ± 0.002

0.623 ± 0.005

43.88 ± 0.31

(b) Model Terms

p-value

  
 

DPPH

MCA

FRAP

TPC

TFC

  

Model

< 0.0001

< 0.0001

< 0.0001

< 0.0001

< 0.0001

  

h

< 0.0001

< 0.0001

< 0.0001

< 0.0001

0.0421

  

h2

0.0011

< 0.0001

< 0.0001

< 0.0001

< 0.0001

  

Lack of fit

0.5135

0.4332

0.7427

0.8416

0.2886

  

R2

0.9874

0.9990

0.9999

0.9999

0.9979

  

Adj. R2

0.9839

0.9998

0.9998

0.9998

0.9973

  

Pred. R2

0.9742

0.9998

0.9998

0.998

0.9967

  

Ad. Prec.

39.33

2365.02

353.46

1197.75

74.71

  

CV (%)

0.94

0.02

0.34

0.04

0.37

  

Std. Dev.

0.37

0.01

< 0.01

< 0.01

0.17

  
  1. Data are presented as mean ± standard deviation (n = 5). h – hypogeal germinated brown rice; R2 – coefficient of determination; Adj. R2- adjusted coefficient of determination; Pred. R2 – predicted coefficient of determination; Ad. Pred. – adequate prediction; CV – coefficient of variation; DPPH - 2, 2-diphenyl-1-picrylhydrazyl; FRAP - ferric reducing powers; MCA - metal chelating activity; TPC - total phenolic content; TFC – total flavonoids content