HPLC peak | Retention time(min) | [M + H(m/z)] | [MS/MS(m/z)] | [Δa.m.u.(m/z)] | Anthocyanin compound | Anthocyanin content (μg/g DW) | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | HAD (40 °C) | HAD (50 °C) | HAD (60 °C) | HAD (70 °C) | FD | MVD | MFD | ||||||
1 | 20.905 | 465.1 | 303 | 162 | Delphinidin-3- O-galactoside | 212.46 ± 3.93a | ND | ND | ND | ND | ND | 28.89 ± 0.40c | 52.85 ± 1.10b |
2 | 22.254 | 465.1 | 303 | 162 | Delphinidin-3- O-glucoside | 16.48 ± 1.06a | ND | ND | ND | ND | ND | 6.88 ± 0.31b | ND |
3 | 23.761 | 449.2 | 287 | 162 | Cyanidin-3- O-galactoside | 175.11 ± 1.11a | ND | ND | ND | ND | 19.43 ± 0.19d | 111.35 ± 0.55c | 128.80 ± 0.13b |
4 | 24.073 | 435.1 | 303 | 132 | Delphinidin-3- O-arabinoside | 155.43 ± 487a | 3.91 ± 0.06e | 2.00 ± 0.06f | 2.01 ± 0.06f | ND | 4.32 ± 0.01d | 29.68 ± 0.07c | 41.48 ± 0.05b |
5 | 25.174 | 449.2 | 287 | 162 | Cyanidin-3- O-glucoside | ND | ND | ND | ND | ND | 14.40 ± 0.11b | 62.86 ± 0.06a | ND |
6 | 25.437 | 479.1 | 317 | 162 | Petunidin-3- O-galactoside | 269.23 ± 7.12a | ND | ND | ND | ND | 7.94 ± 0.35d | 51.99 ± 0.83c | 127.18 ± 1.00b |
7 | 26.752 | 479.1 | 317 | 162 | Petunidin-3- O-glucoside | 178.29 ± 1.52a | 28.77 ± 0.36e | 17.72 ± 0.32f | 17.35 ± 0.02 g | 7.39 ± 0.04 h | 76.05 ± 0.14d | 165.12 ± 0.61b | 127.38 ± 0.42c |
8 | 27.996 | 463.2 | 301 | 162 | Peonidin-3- O-galactoside | 107.31 ± 9.86c | ND | ND | ND | ND | 32.70 ± 0.55d | 112.85 ± 1.86b | 124.48 ± 3.81a |
9 | 28.229 | 449.2 | 317 | 132 | Petunidin-3- O-arabinoside | 158.22 ± 3.31a | 1.69 ± 0.02e | ND | ND | ND | 22.24 ± 0.32d | 71.71 ± 1.49c | 82.26 ± 1.36b |
10 | 29.071 | 493.1 | 331 | 162 | Malvidin-3- O-galactoside | 901.31 ± 22.27a | 158.95 ± 1.49d | 96.91 ± 0.87e | 86.40 ± 0.91f | 35.35 ± 0.05 g | 343.48 ± 1.47c | 630.73 ± 1.11b | 629.75 ± 0.28b |
11 | 30.099 | 463.1 | 301 | 162 | Peonidin-3- O-glucoside | 80.35 ± 2.85b | 23.71 ± 0.02e | 17.76 ± 0.27f | 7.15 ± 0.11 g | 4.47 ± 0.19 h | 48.30 ± 0.48d | 132.55 ± 0.87a | 73.65 ± 0.59c |
12 | 30.442 | 463.2 | 331 | 132 | Malvidin-3- O-arabinoside | 69.40 ± 4.93a | 27.62 ± 0.68e | 21.06 ± 0.34f | 19.83 ± 0.23 g | 12.50 ± 0.24 h | 41.48 ± 0.33d | 59.48 ± 0.76b | 53.45 ± 0.60c |
13 | 31.334 | 493.1 | 331 | 162 | Malvidin-3- O-glucoside | 515.23 ± 26.84a | 204.07 ± 0.90e | 161.59 ± 0.49f | 146.26 ± 0.55 g | 104.35 ± 0.09 h | 303.26 ± 0.19d | 391.31 ± 1.17b | 373.14 ± 0.52c |
14 | 33.676 |  |  |  | Unknown acetyl-anthocyanin |  | ND | ND | ND | ND | 8.87 ± 0.70c | 19.72 ± 0.55a | 13.03 ± 0.47b |
15 | 34.289 |  |  |  | Unknown acetyl-anthocyanin |  | ND | ND | ND | ND | ND | 2.29 ± 0.07a | 2.40 ± 0.30a |
16 | 38.056 |  |  |  | Malvidin-3-O-(6″-acetyl)galactoside |  | 8.42 ± 0.79d | 7.73 ± 0.75e | 5.79 ± 0.69f | 3.25 ± 0.55 g | 25.02 ±  1.64b | 33.09 ± 2.17a | 18.77 ± 1.67c |