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Table 2 The analysis of variance (ANOVA) and estimated regression coefficients of predicted linear and quadratic polynomial models for the responses in the optimization of synbiotic yogurt

From: Functional improvement of synbiotic yogurt enriched with Lacticaseibacillus rhamnosus and aloe vera gel using the response surface method

Coefficients

Physicochemical analysis

Functional analysis

pH

acidity (% w/w)

WHC (% w/w)

Syneresis (% w/w)

Protein content %

Fat content %

Ash content %

Viscosity (cp)

Lab count (log cfu/g)

Antioxidant activity %

Proteolytic activity %

Intercept

3.9

0.833

67.4

39.14

3.29

3.398

0.354

3758.8

7.417

61.1

0.47

X1

-0.2*

0.062

6.5*

-8.48*

-0.1*

-0.1*

-0.002

1694.2*

0.8*

8.17*

0.04*

X2

-0.2*

0.28*

4*

-3.92*

-0.1*

-0.1*

0.012

70.5

-0.6*

4.05*

0.03

X1X2

0

2.13

0

0

0

-0.01

0.008

152.75

0.42*

0

0

X12

0

0.18*

0

0

0

-0.1*

0.017

-348.4

0

0

0

X22

0

-0.07

0

0

0

-0.02

0.027*

-434.4

0

0

0

Model

(p-value)

*

**

***

***

**

***

ns

***

***

***

**

Lack of fit (p-value)

0.5 ns

0.1 ns

0.2 ns

0.05 ns

0.6 ns

0.1 ns

0.3 ns

0.7 ns

0.8 ns

0.5 ns

0.3 ns

R2

0.67

0.90

0.9

0.88

0.64

0.95

0.696

0.968

0.920

0.95

0.54

Coefficients

Functional analysis

Texture analysis

Sensory analysis

inhibition of E.coli %

Inhibition of L.monocytogenes %

Hardness (N)

Gumminess (N)

Adhesiveness (N)

Cohesiveness (N)

Taste

mouthfeel

appearance

Overall acceptability

Intercept

7.60

7.14

35.57

1.13

21.2

0.64

7.48

7.5

7.29

7.46

X1

1.9*

1.67*

-7.3*

-0.5*

-4.2*

0.02

-0.08*

0.05

-0.08*

-0.3*

X2

2.3*

2.5*

0.02

0.21*

-0.41

0.02*

-0.4*

-0.6*

-0.42*

-0.47*

X1X2

0.97*

0

0

0

1.83*

0

0.15*

0.3*

0.15*

-0.05*

X12

0

0

0

0

3.39*

0

-0.2*

0

-0.1*

0.15*

X22

0

0

0

0

-0.08

0

0.073

0

0.1*

0.145*

Model

(p-value)

***

***

***

***

**

***

***

**

***

***

Lack of fit (p-value)

0.2 ns

0.2 ns

0.7 ns

0.1 ns

0.6 ns

0.3 ns

0.2 ns

0.2 ns

0.04 ns

0.2 ns

R2

0.94

0.86

0.92

0.91

0.21

0.78

0.9

0.9

0.98

0.96

  1. X1: storage time. X2: AVG concentrations
  2. * Significant at p ≤ .05. ** Significant at p ≤ .01. *** Significant at p ≤ .001. ns not significant