Table 1 Chromatographic conditions
Sl no | Retention time [min] | Flow rate [mL/min] | Solvent A (%) | Solvent B (%) | Solvent C (%) |
|---|---|---|---|---|---|
1 | 0.000 | 1.000 | 0.0 | 100.0 | 0.0 |
2 | 4.000 | 1.000 | 3.0 | 95.0 | 2.0 |
3 | 10.000 | 1.000 | 6.0 | 92.0 | 2.0 |
4 | 14.000 | 0.800 | 6.0 | 90.0 | 4.0 |
5 | 20.000 | 0.800 | 10.0 | 85.0 | 5.0 |
6 | 24.000 | 0.750 | 14.0 | 80.0 | 6.0 |
7 | 30.000 | 0.750 | 15.0 | 75.0 | 10.0 |
8 | 39.000 | 0.750 | 20.0 | 65.0 | 15.0 |
9 | 45.000 | 0.750 | 25.0 | 55.0 | 20.0 |