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Table 3 Box-Behnken experiment design matrix and results

From: Production and characterization of bacterial cellulose from kombucha-fermented soy whey

Trial

Variablea

Response

X1

X2

X3

Resulted BC dry wt (g/100 mL)

Predicted BC dry wt (g/100 mL)

1

0

-1

1

1.02

1.10

2

0

0

0

4.48

4.17

3

0

0

0

4.00

4.17

4

0

1

-1

3.20

3.12

5

0

0

0

4.20

4.17

6

-1

0

1

1.24

1.00

7

-1

1

0

3.63

3.80

8

-1

0

-1

3.13

3.04

9

1

1

0

3.84

3.68

10

-1

-1

0

3.12

3.28

11

1

0

-1

2.87

3.11

12

0

0

0

3.96

4.17

13

1

-1

0

4.05

3.88

14

0

1

1

1.18

1.25

15

0

0

0

4.19

4.17

16

1

0

1

1.35

1.44

17

0

-1

-1

3.02

2.95

  1. Variable a: X1-X3 (variable to be optimized) are sucrose addition (%), kombucha inoculation amount(%), fermentation temperature (℃), respectively; the coded low, middle and high level are presented as − 1, 0 and 1, respectively