Nutritional, phytochemical and antioxidant properties of 24 mung bean (Vigna radiate L.) genotypes

Abstract

This study aimed to investigate the proximate and phytochemicals present in seeds of 24 mung bean (Vigna radiate L.) genotypes from four provinces of China for estimating their nutritional and antioxidant properties. Proximate analysis of mung bean genotypes revealed that starch, protein, fat, ash and water-soluble polysaccharide ranged from 39.54–60.66, 17.36–24.89, 4.24–12.18, 2.78–3.53 and 1.99–2.96 g/100 g respectively. The five principal fatty acids detected in mung beans were stearic acid, palmitic acid, linoleic acid, oleic acid, and linolenic acid. The contents of insoluble-bound phenolic compounds, soluble phenolic compounds, and flavonoids ranged from 0.78 to 1.5 mg GAE g^− 1, 1.78 to 4.10 mg GAE g^− 1, and 1.25 to 3.52 mg RE g^− 1, respectively. The black seed coat mung bean genotype M13 (Suheilv 1) exhibited highest flavonoid and phenolic contents which showed strong antioxidant activity. Two flavonoids (vitexin and isovitexin) and four phenolic acids (caffeic, syringic acid, p-coumaric, and ferulic acids) were identified by HPLC. Vitexin and isovitexin were the major phenolic compounds in all mung bean genotypes. The content of soluble phenolic compounds had positive correlation with DPPH (r² = 0.713) and ABTS (r² = 0.665) radical scavenging activities. Principal component analysis indicated that the first two principal components could reflect most details on mung bean with a cumulative contribution rate of 66.1%. Twenty-four mung bean genotypes were classified into four groups based on their phenolic compounds contents and antioxidant activities. The present study highlights the importance of these mung bean genotypes as a source of nature antioxidant ingredient for the development of functional foods or a source of health promoting food.

Graphical Abstract

Introduction

Mung bean (Vigna radiate L.) is a species of Fabaceae plant which is well-known as green gram (Ganesan & Xu 2018). It has been widely grown in the Southeast Asia and is very common in consumer products around the world. Mung beans are rich in protein, starch, cellulose, minerals, and vitamins (Khaket et al. 2015). Studies have shown that mung bean has physiological functions such as anti-obesity, anti-oxidation, and anti-bacterial (Yao et al., 2013). Mung bean provides a high-quality natural plant protein source which has been used as a substitute for meat and milk protein in many underdeveloped countries (Connolly et al. 2015; Du et al. 2018). Studies have shown that mung bean polysaccharide has antioxidant and immunomodulatory activities (Lai et al. 2010). Moreover, the essential fatty acids (FAs) contained in mung beans can promote the growth and development of the body. In addition to its nutritional importance, mung beans are a rich source of phytochemicals including phenols and flavonoids that show health promoting effects such as antioxidants, anti-tumor and anti-radiation (Randhir & Shetty 2007; Soucek et al. 2005). Phenolic phytochemicals are the largest category of phytochemicals and the most widely distributed in plant (King & Young 1999). They mainly exist in free, soluble bound and insoluble bound forms (Alshikh et al. 2015; Jung et al. 2002).

Mung beans are commonly consumed food legume in Asian countries. Seeds of mung beans have been used to prepare a variety of fresh, fermented and dried foods. They are very popular foods in China and are known as “green pearls.” Mung beans are often used in food processing, such as mung bean porridge, mung bean soup, bean paste, mung bean cake, and raw bean sprouts. They can also be used as jelly, noodles, and vermicelli due to their high starch content. In addition, they are good raw materials for famous wines. Mung bean protein has excellent functional properties such as solubility, water retention, emulsification, gelation, foaming, and foam stability. It has application prospects in flour products, meat products, dairy products, and beverages in the food processing industry. Protein beverages (protein milk, coffee bean milk) made from protein isolates have high nutritional value and high-quality.

Following a country-wide collection during the 1980′s, more than 5000 accessions of mung bean germplasm have been deposited in the National Crop Genebank of China (Liu et al. 2006; Wang et al. 2018). In despite of the abundant germplasm resources of mung bean, the diversity of nutritional composition is still unknown. Due to importance of mung bean and its products, it is necessary to investigate nutritional and phytochemical compounds across various genotypes. In this study, 24 mung beans genotypes from four provinces in China were collected for the following purposes: (1) compare their nutritional compositions, (2) analyze their phytochemicals contents, and (3) evaluate correlations between phytochemical compounds and antioxidant activities. Results of this study will provide a good basis for the assessment and application of different mung bean genotypes.

Materials and methods

Materials

Twenty-four mung bean genotypes (Fig. 1) were collected from four provinces: Shanxi (north of China), Shaanxi (northwest of China), Jiangsu (east of China), and Hebei (north of China). We selected six mung bean genotypes per province. Among them, M12-M18 genotypes come from our lab. Name and geographical origin of these genotypes are presented in Table 1. Seeds of these genotypes were grown in the Liuhe Base of Jiangsu Academy of Agricultural Sciences.

Fig. 1

Photos of 24 mung bean genotypes (M1–24)

Table 1 Name and geographical origin of the 24 mung bean genotypes

2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), ascorbic acid (vitamin C, Vc), Folin–Ciocalteu’s phenol reagent were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). All other reagents used were of analytical grade and purchased from Sodebio Reagent Co., Ltd. (Nanjing, Jiangsu, China).

Analysis of nutrient components

Protein content was determined by using Kjeldahl method with Kjeltec TM2300 Auto Sampler System (Foss Analytical, Hillerød, Denmark) (Thiex et al. 2002). Soxhlet extraction (Extraction System B-811, Buchi, Flawil, Sankt Gallen, Switzerland) with petroleum ether was used to determine crude fat content (AOAC 1990). Ash content was determined by using the combustion method with an electric muffle furnace (SX2–4-13, Leiyun Instruments, Shanghai, China) (AOAC 1990). Starch content was determined using a starch kit (BC0700, Solarbio Science & Technology Co., Ltd., Beijing, China).

Fatty acid composition

Fatty acid (FA) composition was determined according to the method of Zhang et al. (2013) with slight modifications. Briefly, 0.5 g mung bean powder was added in a 2 mL centrifuge tube with 1.5 mL n-hexane. The mixture was left overnight and then centrifuged at 8200 g for 5 min (Eppendorf Centrifuge 5804R, Hamburg, Germany). Then, 350 μL of sodium methoxide solution was added and vortexed for 1 h. After centrifugation, the supernatant was as used for high-performance gas chromatography analysis (Agilent 7890B, Agilent Technologies Inc., Wilmington, DE, USA).

Determination of water-soluble polysaccharide (WSP)

The WSP was determined according to Yao et al. (2016) with several modifications. The mung bean powder was extracted with 80% ethanol for 1 h and then centrifuged at 2000 g for 5 min. The supernatant was removed and extracted twice with distilled water at 90 °C for 3 h. After centrifugation, the supernatant was collected with Sevag reagent (chloroform:n-butanol = 4:1,v/v) and shaken for 10 min. The mixture was centrifuged at 2000 g for 5 min. The lower organic solvent and protein at the interface were then removed. The gelatin was denatured, and the upper polysaccharide solution was retained.

Preparation of standard curve: 10 mg of glucose standard solution was accurately weighed and dissolved in 10 mL to obtain 1 mg/mL glucose standard solution. The solution was diluted to 10, 20, 40, 60, 80, and 100 μg/mL. Then, 1.0 mL of the diluted solution was drawn, and 1 mL of distilled water was added. Immediately thereafter, 1.0 mL of a 6% phenol solution was added, and 5.0 mL of concentrated sulfuric acid was slowly added and shaken while adding. After mixing well, the solution was left to stand at room temperature for 20 min. The absorbance of the solution was measured at 490 nm by a spectrophotometer (Alpha-1101 m, Puyuan Instruments, Shanghai, China). Next, 1.0 mL of polysaccharide sample solution with certain concentration was drawn, and distilled water was added to make it 2.0 mL. The subsequent operation was the same as glucose labeling. The purity of the corresponding polysaccharide was calculated according to its absorbance value and standard curve.

Extract analysis

Preparation of ethanol extracts

Mung bean powder (1 g) was mixed with 25 mL of 80% ethanol solution and extracted in a water bath for 4 h. The solution was cooled to room temperature and centrifuged at 15000 g for 15 min. The operation was repeated 2–3 times, and the obtained supernatant was mixed and concentrated. The volume was made to reach 20 mL with methanol and stored at − 20 °C for testing. The collected supernatants were examined for soluble phenolic compounds.

Determination of total flavonoid content

Total flavonoid content (TFC) was determined according to the method reported by Xie et al. (2015). The sample extract (250 μL) was mixed with 1.25 mL of distilled water, and 25 μL of 5% NaNO₂ was added to react for 6 min. Then, 150 μL of 10% AlCl₃ was added to react for 5 min. Finally, 0.5 mL of 1 M NaOH and 275 μL of distilled water were added to the mixture, and the absorbance was measured at 510 nm after 10 min. Rutin with different concentrations was plotted as the standard curve, and the results were expressed as rutin equivalents (RE).

The content of soluble phenolic compounds

The content of soluble phenolic compounds in the samples was determined by using the Folin-Ciocalteu method. The sample solution (400 μL) and 4.6 mL of deionized water were placed in a test tube, and 1 mL of Folin-Ciocalteu and 24 mL of 7.5% (w/v) of sodium carbonate solution were injected into the test tube. After reacting for 2 h at room temperature, the absorbance of the sample was measured at a wavelength of 760 nm. The standard curve was configured with different concentrations of gallic acid, and the results were expressed as gallic acid equivalents (GAE).

The content of insoluble-bound phenolic compounds

Extraction of insoluble-bound phenolic compounds was performed according to the method reported by Zhang et al. (2013). Briefly, 20 mL of NaOH (2 mol/L) was mixed with the residue left after extracting soluble phenolic compounds and incubated for 1 h at room temperature. The mixture was centrifuged at 4500 g for 5 min, and the supernatant was extracted and adjusted to pH 2 with HCl (6 mol/L). Then, an equal volume of ethyl acetate was added for extraction, and the extraction was repeated 3 times. The extract was mixed, evaporated to dryness by a rotary evaporator at 45 °C, reconstituted to 5 mL of methanol, and determined by using the Folin-Ciocalteu method.

High-performance liquid chromatography (HPLC) analysis of phenolic compounds

Agilent 1260 high performance liquid chromatography (Agilent Technologies Inc. Santa Clara, CA, USA) equipped with the Agilent Poroshell 120 EC-C18 Column was used to detect the main phenolic compounds in mung bean seeds. The mobile phase consisted of acetonitrile (A) and ultrapure water containing 0.1% trifluoroacetic acid (B). Gradient elution was performed as follows: 0–7 min, 5–30% A; 7–15 min, 30–40% A; 15–25 min, 40–50% A; 25–30 min, 50–95% A; 30–35 min, 95–5% A. The flow rate was set at 1.0 mL min^− 1 and the injection volume was 10 μL. The column was operated at 30 °C. The wavelength of the detector was set at 280 nm. Quantification of phenolic compounds was carried out by an external standard method using calibration curves.

Evaluation of antioxidant capacity

Assay of DPPH radical scavenging activity

DPPH analysis was executed in line with the report of Chai et al. (2018). Briefly, DPPH was dissolved in methanol. Then, 0.5 mL of DPPH (0.4 mmol/L) and 0.5 mL of the extract were mixed to react in the dark at room temperature for 30 min. The absorbance was measured at 517 nm with a spectrophotometer. The results were expressed as μmol vitamin C per gram samples.

Assay of ABTS radical cation scavenging activity

ABTS radical cation scavenging assay was performed according to the method reported by Lee et al. (2011) with some modifications. ABTS radical ions were produced by mixing 7 mM ABTS aqueous solution with 2.45 mM K₂S₂O₈ aqueous solution, storing in the dark for 16 h, diluting 20 times with absolute ethanol before use, and storing at 30 °C. Next, 1.2 mL of ABTS ethanol solution was mixed with 300 μL of extract to react at 30 °C for 6 min. The absorbance was measured at 734 nm with a spectrophotometer. The results were expressed as μmol vitamin C per gram.

Hydroxyl radical scavenging ability

The hydroxyl radical scavenging ability was determined according to Xiao et al. (2015) with simple modifications. Briefly, 300 μL of FeSO₄ (9 mmol/L), 300 μL of H₂O₂ and 300 μL of the extract were mixed. After shaking, the mixture was added with 300 μL of salicylic acid-ethanol (9 mmol/L) and incubated at 37 °C for 30 min. The absorbance was measured at 510 nm. The results were expressed as μmol vitamin C per gram samples.

Statistical analysis

All experiments were conducted in triplicate and results were expressed as mean ± standard deviation. The statistical significance of the results was obtained by one-way analysis of variance and PCA using SPSS version 21.0 software. The Pearson correlation coefficient was used to estimate the correlation between phytochemical compounds and antioxidant activities. PCA was used to assess the contribution of chemical components to mung beans.

Results and discussion

Nutritional compositions

Protein and starch are the two most abundant nutritional components in mung bean seed. In this study, the protein and starch contents of 24 mung bean genotypes ranged from 17.36 to 24.89 g/100 g and 39.54 g/100 g to 60.66 g/100 g (Table 2), respectively. M6 (Jinlv 399) showed the highest contents of protein (24.89 g/100 g) and starch (60.66 g/100 g) which could be used for specific food processing, such as noodles and vermicelli. Compared with other legumes, mung beans have a higher carbohydrate content, predominantly starch (Tang et al. 2014). The contributions of ash were in the ranges of 2.78 to 3.53 g/100 g. The content of water-soluble polysaccharide (WSP) ranged from 1.99 to 2.96 g/100 g. M14 (Sulv 3) exhibited the highest content of WSP, while M2 showed the lowest value. The biological activity of polysaccharides has received attention, such as antioxidant and immunological activities.

Table 2 Proximate analysis of different mung bean genotypes

Table 2 shows the crude fat content and FA composition of 24 kinds of mung beans. The content of crude fat ranged from 4.24 to 12.18 mg/g. High-performance gas chromatography was used for the analysis of FA in mung bean. Five principal FAs were observed and identified according to standards (Fig. 2). Peaks 1, 2, 3, 4 and 5 with the retention time of 2.402, 3.187, 3.318, 3.602 and 3.953 min were referred to as palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid, respectively. Linoleic acid was the most abundant in mung bean and accounted for 38.95–44.74 percentage of the total fatty acids. A similar observation was reported by Anwar et al. (2007) who investigated fatty acid composition of different mung bean cultivars grown in Pakistan. Studies have shown that polyunsaturated fatty acids are important structural substances in the retina and neurons which can protect the vision. Polyunsaturated fatty acids have important physiological functions in human metabolism, such as esterification of cholesterol, lowering blood cholesterol and triglycerides (Yates et al. 2014).

Fig. 2

High performance gas chromatogram of fatty acids in mung bean. Peaks 1, 2, 3, 4 and 5 were identified as palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid

Contents of phytochemicals (total flavonoid content, insoluble-bound phenolic content and soluble phenolic content)

As shown in Table 3, the total flavonoid content of 24 mung bean genotypes ranged from 1.25 to 3.52 mg RE g^− 1. M13 (Suheilv 1) presented the highest flavonoid content (3.52 mg RE g^− 1) and M5 (Jinlv 995) presented the lowest flavonoid content. Flavonoids are general name of a group of chemicals including catechins, anthocyanidins, proanthocyanidins, flavonols, isoflavonoids and flavones (Luo et al. 2016). Flavonoids have important physiological functions such as anti-oxidation and anti-inflammatory (Zuk et al. 2019). Zhang et al. (2013) used acetone and water as extraction solvents, and the highest flavonoid content obtained was 6.0 mg g^− 1. This may be different from the extraction solvent. The nature of the raw materials itself also has a relationship, such as the difference in the color of the skin and the difference in maturity.

Table 3 Contents of phytochemicals of different mung bean genotypes

The contents of soluble and insoluble-bound phenolic compounds in 24 mung bean genotypes investigated are shown in Table 3. The phenolic content of the soluble fraction ranged from 1.78 to 4.10 mg GAE g^− 1. Insoluble-bound phenolic content ranged from 0.78 to 1.5 mg GAE g^− 1. M13 genotype exhibited the highest content of both soluble and insoluble-bound phenolic compounds. Meanwhile, M22 had the lowest soluble phenolic content of 1.78 mg GAE g^− 1 and M8 exhibited the lowest insoluble-bound phenolic content of 0.78 mg GAE g^− 1. The difference between soluble and insoluble-bound phenolic contents is significant, which is similar to Wang et al. (2016). A similar result was reported by de Camargo et al. (2015) who observed that the content of soluble phenolic compounds (free and esterified) was significantly higher than insoluble-bound fraction in peanut skin. The amount of phenolic compound is affected by genotype, agronomic habits (irrigation, fertilization, and pest management), harvest maturity, post-harvest storage, and climatic conditions (Mattila et al. 2005).

Identification of major phenolic compounds in mung bean seeds

Soluble and insoluble-bound phenolic compounds in mung bean seeds were identified by HPLC as shown in Fig. 3 and Table 4. Peaks 1, 2, 3, 4, 5 and 6 with the retention time of 17.785, 18.534, 24.160, 29.017 and 29.731 min were referred to as caffeic acid, syringic acid, p-coumaric acid, ferulic acid, vitexin and isovitexin, respectively (Fig. 3). In the present study, six phenolic compounds were identified, including four phenolic acids (syringic, caffeic, p-coumaric, and ferulic acids) and two flavonoids (vitexin and isovitexin). Results showed that vitexin and isovitexin were the dominant phenolic compounds in all mung bean genotypes. This is consistent with a previous study (Yang et al. 2020). The vitexin content of soluble and insoluble-bound fractions ranged from 481.02 to 910.26 μg g^− 1 and from 123.77 to 463.25 μg g^− 1, respectively. The isovitexin content of soluble and insoluble-bound fractions ranged from 568.57 to 1572.74 μg g^− 1 and from 104.42 to 421.77 μg g^− 1, respectively. The isovitexin content in the soluble fractions of mung bean seeds were higher than their corresponding vitexin content. M13 showed the highest vitexin (910.26 μg g^− 1) and isovitexin (1572.74 μg g^− 1) contents in the soluble fractions of all studied mung beans. Large differences were found among all mung beans in the contents of both soluble and insoluble-bound flavonoids. The vitexin and isovitexin contents in the soluble fractions of mung beans are higher than that in the insoluble-bound fractions. The contents of individual phenolic acids in different bean varieties are also shown in Table 4. Contrary to flavonoids, the contents of phenolic acids in the soluble fractions are lower than that in the insoluble-bound fractions. Shi et al. (2016) also reported that the average content of bound phenolic acids in the mung bean samples accounted for 89.8% of the total amount of phenolic acids. Insoluble-bound phenolic compounds can survive upper gastrointestinal digestion and are released from the colon by the effect of microorganism. Results suggested that caffeic acid was the major phenolic acid in mung bean cultivars, ranged from 2.45 to 15.12 μg g^− 1 in the soluble fractions and from 2.38 to 16.46 μg g^− 1 in the insoluble-bound fractions, respectively. Caffeic acid is an effective scavenger of the ABTS⁺ and DPPH radicals, which has strong antioxidant activity (Gülin 2006). Syringic acid was not detected in all mung bean varieties except M18. Flavonoids and phenolic acids are regarded as the major compounds contributing to the total antioxidant activities of mung bean seeds (Shi et al. 2016).

Fig. 3

High-performance liquid chromatogram of phenolic compounds in mung bean. Peaks 1, 2, 3, 4, 5 and 6 were identified as caffeic acid, syringic acid, p-coumaric acid, ferulic acid, vitexin and isovitexin

Table 4 Phenolic compounds identified in mung bean seeds

Antioxidant activity

The results of antioxidant activities are presented in Fig. 4. Soluble phenolic compounds showed stronger antioxidant activity than insoluble-bound phenolic compounds. M23 (Jilv 9025) exhibited the strongest DPPH free radical scavenging ability (Fig. 4a) for soluble phenolic compounds (4.44 μmol/g). At the same time, M13 had the strongest DPPH free radical scavenging ability for the insoluble-bound fraction (3.73 μmol/g). The antioxidant activity can be influenced by many factors and cannot be fully described with one single method. These commonly used methods have their advantages and disadvantages for measuring antioxidant activity. Therefore, in this study, the radical scavenging performance was also evaluated by using the ABTS radical cation and hydroxyl radical assays. Regardless of the soluble or insoluble-bound phenolic compounds, M13 (Suheilv 1) genotype showed the highest ABTS radical scavenging ability (Fig. 4b). The result showed that the ability of soluble phenolic compounds to scavenge hydroxyl radicals ranged from 10.38 to 15.54 μmol/g (Fig. 4c). However, the insoluble-bound phenolic compounds exhibited significantly lower hydroxyl radical scavenging activity. Free radicals will seize the electrons of biomolecules, causing the biomolecules to be altered and cause various diseases, such as inflammation, aging, and cardiovascular diseases. Phenolic compounds can act as hydrogen or electron donors when reacting with oxidative substances (Luo et al. 2016). Therefore, they present strong free radical scavenging activity and antioxidant activity. In general, results indicated that the black seed coat mung bean genotype M13 from our lab presented the strongest antioxidant activity.

Fig. 4

Antioxidant activities of different mung bean genotypes (mean ± SD, n = 3), a DPPH radical scavenging activity; b ABTS radical cation scavenging activity; c hydroxyl radical scavenging ability. Results were expressed as μmol VC/g

Correlation of antioxidant activity with the contents of soluble phenolic compounds and flavonoids

As shown in Table 5, soluble phenolic content has significantly high correlation with DPPH (r² = 0.713) and ABTS (r² = 0.665) radical scavenging activities. Flavonoid content is significantly correlated with DPPH (r² = 0.463) radical scavenging activity. No evident correlation is observed between hydroxyl radical scavenging ability and phytochemical contents. A high correlation between the content of phenolic compounds and antioxidant activity has also been previously demonstrated by Shi et al. (2016).

Table 5 Correlation analysis between antioxidant activity and phytochemicals contents

Principal component analysis

Data of phenolic compounds content, flavonoid content and antioxidant activities of the 24 mung bean genotypes were subjected to principal component analysis (PCA). As shown in Fig. 5, the first two principal components explained 66.1% of the total variation (R2X [1] =37.9% and R2X [2] =28.2%). The PCA scatter plot revealed the dispersion between the 24 mung bean genotypes. M13 genotype had the largest deviation from the other genotypes, presenting the highest antioxidant activity, phenolic compounds content and flavonoid contents. The longest distance between M13 and M8 showed significant differences in terms of phytochemical contents and antioxidant activities. Principal component analysis indicated that although different mung beans had similar growth environment, they could show significantly differences in phytochemical and antioxidant properties because of their different genotypes. The 24 mung beans were classified into four groups. Group 1 was characterized by high levels of TFC, phenolic contents, DPPH and ABTS free radical scavenging abilities, which contained only M13. Group 2 contained M4, M5, M15, M18 and M22. These genotypes mainly presented similar antioxidant activity. Results of PCA showed satisfactory separation of phenolic compounds and antioxidant activity of these genotypes, indicating that M13 is significantly different from other mung bean genotypes, and implying its potential nutritional and functional values in food processing. Results of this study could provide a good reference for the selection of mung bean genotypes in food production and processing.

Fig. 5

Principal component analysis based on the phenolic compounds content, flavonoid content and antioxidant activities of 24 mung bean genotypes

Conclusions

In this study, the nutritional composition, phytochemicals contents and correlations between phytochemical compounds and antioxidant activities of 24 mung bean genotypes from four provinces in China were investigated. The nutritional composition and phytochemical properties of 24 mung bean genotypes are different from each other. Starch, protein, fat, ash and water-soluble polysaccharide ranged from 39.54 to 60.66, 17.36 to 24.89, 4.24 to 12.18, 2.78 to 3.53 and 1.99 to 2.96 g/100 g respectively. M13 (Suheilv 1) showed the highest content of phytochemicals contents, such as flavonoids (3.52 mg RE g^− 1), soluble phenolic compounds (4.10 mg GAE g^− 1), and insoluble-bound phenolic compounds (1.50 mg GAE g^− 1). Vitexin and isovitexin were identified by HPLC as the major phenolic compounds in all mung bean genotypes. M13 showed the highest vitexin (910.26 μg g^− 1) and isovitexin (1572.74 μg g^− 1) contents in the soluble fractions of all studied mung beans. Different assays were performed to judge the antioxidant activity of mung bean genotypes, and M13 exhibited strong DPPH and ABTS radical scavenging activities. According to principal component analysis, 24 mung bean samples were classified into four groups based on their phenolic compounds contents and antioxidant activity. The black seed coat mung bean genotype M13 could be used as a superior variety with high antioxidant capacity for functional food production and processing. Overall, this work provides useful information for the potential future application of different mung bean genotypes as source of functional and healthy food. More intensive studies are needed to identify the most effective chemical components in these investigated genotypes.